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dc.contributor.authorMartinez-Fleta, Pedroes_ES
dc.contributor.authorAlfranca, Arántzazues_ES
dc.contributor.authorGonzález-Álvaro, Isidoroes_ES
dc.contributor.authorCasasnovas, José Maríaes_ES
dc.contributor.authorFernández-Soto, Danieles_ES
dc.contributor.authorEsteso, Gloriaes_ES
dc.contributor.authorCáceres-Martell, Yaizaes_ES
dc.contributor.authorGardeta, Sofíaes_ES
dc.contributor.authorPrat, Salomées_ES
dc.contributor.authorMateu-Alberoa, Tamaraes_ES
dc.contributor.authorGabrie, Ligiaes_ES
dc.contributor.authorLópez-Granados, Eduardoes_ES
dc.contributor.authorSánchez-Madrid, Franciscoes_ES
dc.contributor.authorRodríguez-Frade, José Migueles_ES
dc.contributor.authorReyburn, H. T.es_ES
dc.contributor.authorValés-Gómez, Mares_ES
dc.date.accessioned2020-08-11T08:30:57Z-
dc.date.available2020-08-11T08:30:57Z-
dc.date.issued2020-07-18-
dc.identifier.urihttp://hdl.handle.net/10261/217715-
dc.description.abstractCurrently, there is a need for reliable tests that allow identification of individuals that have been infected with SARS-CoV-2 even if the infection was asymptomatic. To date, the vast majority of the serological tests for SARS-CoV-2 specific antibodies are based on serum detection of antibodies to either the viral spike glycoprotein (the major target for neutralising antibodies) or the viral nucleocapsid protein that are known to be highly immunogenic in other coronaviruses. Conceivably, exposure of antigens released from infected cells could stimulate antibody responses that might correlate with tissue damage and, hence, they may have some value as a prognostic indicator. We addressed whether other non-structural viral proteins, not incorporated into the infectious viral particle, specifically the viral cysteine-like protease, might also be potent immunogens. Using ELISA tests, coating several SARS-CoV-2 proteins produced in vitro, we describe that COVID-19 patients make high titre IgG, IgM and IgA antibody responses to the Cys-like protease from SARS-CoV-2, also known as 3CLpro or Mpro, and it can be used to identify individuals with positive serology against the coronavirus. Higher antibody titres in these assays associated with more severe disease and no cross-reactive antibodies against prior betacoronavirus were found. Remarkably, IgG antibodies specific for Mpro and other SARS-CoV-2 antigens can also be detected in saliva. In conclusion, Mpro is a potent antigen in infected patients that can be used in serological tests and its detection in saliva could be the basis for a rapid, non-invasive test for COVID-19 seropositivity.es_ES
dc.description.sponsorshipThis work was supported by the Spanish National Research Council (CSIC, project number 202020E079) and grants from Madrid Regional Government IMMUNOTHERCAN [S2017/BMD-3733-2 (MVG)]; the Spanish Ministry of Science and Innovation [(MCIU/AEI/FEDER, EU): RTI2018-093569-B-I00 (MVG), SAF2017-82940-R (JMRF), SAF2017-83265-R (HTR); SAF2017-82886-R (FSM)]; RETICS Program of ISCIII [RD16/0012/0006; RIER (JMRF); RD16/0011/0012, PI18/0371 (IGA), PI19/00549 (AA)]. The study was also funded by La Caixa Banking Foundation (HR17-00016 to FSM) and Fondo Supera COVID (CRUE-Banco de Santander) to FSMes_ES
dc.language.isoenges_ES
dc.publisherMedRxives_ES
dc.relationS2017/BMD-3733-2/IMMUNOTHERCANes_ES
dc.relationMICIU/ICTI2017-2020/RTI2018-093569-B-I00es_ES
dc.relationRTI2018-093569-B-I00/AEI/10.13039/501100011033es_ES
dc.relationMICIU/ICTI2017-2020/SAF2017-82940-Res_ES
dc.relationSAF2017-82940-R/AEI/10.13039/501100011033es_ES
dc.relationMICIU/ICTI2017-2020/SAF2017-83265-Res_ES
dc.relationSAF2017-83265-R/AEI/10.13039/501100011033es_ES
dc.relationMICIU/ICTI2017-2020/SAF2017-82886-Res_ES
dc.relationSAF2017-82886-R/AEI/10.13039/501100011033es_ES
dc.relation.isversionofPreprintes_ES
dc.rightsopenAccesses_ES
dc.subjectCoronaviruses_ES
dc.subjectDiagnosticses_ES
dc.subjectSerologyes_ES
dc.subjectCOVID-19es_ES
dc.subjectELISAes_ES
dc.titleSARS-Cov-2 cysteine-like protease (Mpro) is immunogenic and can be detected in serum and saliva of COVID-19-seropositive individualses_ES
dc.title.alternativeSARS-CoV-2 protease antibodies in serum and salivaes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1101/2020.07.16.20155853-
dc.description.peerreviewedNoes_ES
dc.relation.publisherversionhttps://doi.org/10.1101/2020.07.16.20155853es_ES
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderObra Social la Caixaes_ES
dc.contributor.funderConferencia de Rectores de las Universidades Españolases_ES
dc.contributor.funderBanco Santanderes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100010784es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.contributor.orcidCasasnovas, José María [0000-0002-2873-6410]es_ES
dc.contributor.orcidReyburn, H. T. [0000-0003-2855-1595]es_ES
dc.contributor.orcidValés-Gómez, Mar [0000-0001-7424-3206]es_ES
Appears in Collections:(CNB) Artículos
(VICYT) Colección Especial COVID-19
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