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Title

Separation, determination and composition profile of lipids in biodiesel using hyphenation of gradient HPTLC with fluorescence detection by intensity changes and mass spectrometry

AuthorsJarne, Carmen; Cebolla, Vicente L. ; Membrado, Luis; Lapieza Remón, Mª Pilar; Savirón, María; Orduna, Jesús
KeywordsBiodiesel
HPTLC
Lipids
Issue Date16-Aug-2015
Citation250th ACS National Meeting (2015)
AbstractWe report here an on-line, hyphenated High-Performance Thin-Layer Chromatography (HPTLC)-based procedure on a unique silica gel plate that provides separation and quantitative determination of monoglycerides, diglycerides, and fatty acids in biodiesel (BX, X>5), as well as their composition profile in the sample. Sequential steps of hyphenation include: AMD gradient separation of lipid classes (using a 4-step gradient based on t-butyl methyl ether-dichloromethane-n-heptane; followed by a post-chromatographic impregnation of the plate with primuline (200 ppm), and densitometric detection and quantification using Fluorescence Detection by Intensity Changes (FDIC, >exc= 365 nm). Finally, the peaks are directly transferred from the impregnated plate to an ESI-MS spectrometer using a head elution-based TLC-MS interface for peak identification and obtention of fatty acid-related composition profiles of each lipid class
DescriptionPóster presented at the 250th ACS National Meeting, Boston (USA), 2015, 16th-20th august.
URIhttp://hdl.handle.net/10261/217688
ReferencesJarne, Carmen
Cebolla, Vicente L.
Membrado, Luis
Lapieza Remón, Mª Pilar
Savirón, María
Orduna, Jesús; Separation, determination and composition profile of lipids in biodiesel using hyphenation of gradient HPTLC with fluorescence detection by intensity changes and mass spectrometry; http://hdl.handle.net/10261/148050
Appears in Collections:(ICMA) Comunicaciones congresos
(ICB) Comunicaciones congresos
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