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White storks (Ciconia ciconia), landfills and extendedspectrum beta-lactamases-producing Escherichia coli

AuthorsHöfle, Ursula ; Camacho, MariaCruz; Puente, Javier de la; Pineda-Pampliega, Javier; Aguirre, Jose Ignacio; Torres, Fernando; Blas, Julio ; Ramis, Antoni; Majó, Natàlia; Migura, Lourdes; Hernández, José Manuel
Issue Date2019
Citation68th Annual International Conference of the Wildlife Disease Association (2019)
AbstractWhite storks (Ciconia ciconia) are large birds that connect Europe, Africa and parts of Asia through their migratory flyways. Recent studies on the movement ecology of storks report ample effects of landfills on stork ecology, life histories and behavior. The combination of epidemiology and movement ecology tools offers unique possibilities to understand how zoonotic diseases may spread, the location of potential sources and risk areas. Between 2011 and 2014, we sampled white stork nestlings from colonies along a gradient of exposure to landfill foraged food, with the objective of assessing the potential of the species to act as reservoir and vehicle of cephalosporin resistant (CR) Escherichia coli. E. coli isolates were phenotypically characterized, presence of CR genes was confirmed and plasmids were classified. Risk factors for acquiring these genes were assessed. Overall, 8.8% (41 out of 467) storks carried CR E. coli in their cloaca and five were identified from recently deposited droppings; therefore 46 isolates were further characterized. Of these, 21 contained blaCTX-M-1, 10 blaCMY-2, 6 blaCTX-M-14, 3 blaSHV-12, 3 blaCTX-M-15, 2 blaCTX-M-1 together with blaCMY-2, and 1 blaCTX-M-1 together with blaSHV-12. All were multi-resistant, and three harboured the plasmid-mediated colistin resistance mcr-1 gene. CR genes were associated with the presence of IncI1, IncFIB and IncN replicon families. Pulsed-field gel electrophoresis demonstrated a high degree of polymorphism, but showed also identical profiles from isolates obtained from different locations. Carriage of CR E. coli was strongly associated to use of landfills. This study demonstrates that the proximity of white storks to human activities with high antimicrobial pressure contributes to the acquisition and dissemination of CR E. coli.
DescriptionResumen del trabajo presentado a la 68th Annual International Conference of the Wildlife Disease Association (WDA): "Fostering resiliency in a time of change", celebrada en Tahoe City, California (USA) del 4 al 9 de agosto de 2019.
Appears in Collections:(EBD) Comunicaciones congresos
(IREC) Comunicaciones congresos
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