English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/217363
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Calbindin immunostaining in the CA1 hippocampal pyramidal cell layer of the human and mouse: A comparative study

AuthorsMerino-Serrais, Paula ; Tapia-González, Silvia; DeFelipe, Javier
KeywordsCalcium binding proteins
Pyramidal neuron
Non-pyramidal neuron
Postmortem time delay
Species differences
Issue Date2020
CitationJournal of Chemical Neuroanatomy 104 (2020)
AbstractImmunostaining for calbindin (CB) is commonly used to label particular populations of neurons. Recently, it has been shown that the CA1 pyramidal cells in the mouse can be subdivided along the radial axis into superficial and deep pyramidal cells and that this segregation in the radial axis may represent a general principle of structural and functional organization of the hippocampus. One of the most widely used markers of the superficial pyramidal cells is CB. However, this laminar segregation of pyramidal cells has not been reported in the human CA1 using CB immunostaining. The problem is that the different pattern of CB immunostaining observed in the mouse compared to the human could be explained by technical features, of which one of the most important is the postmortem time (PT) delay typical of the brain tissue obtained from humans. In the present study, we have studied the influences of PT delays and fixation procedures and we found that the clear differences found between the CA1 of the human and mouse do not depend on the fixation, but represent actual species-specific differences. These remarkable differences between species should be taken into consideration when making interpretations in translational studies from mouse to human brains.
Publisher version (URL)http://dx.doi.org/10.1016/j.jchemneu.2020.101745
URIhttp://hdl.handle.net/10261/217363
DOIhttp://dx.doi.org/10.1016/j.jchemneu.2020.101745
Identifiersdoi: 10.1016/j.jchemneu.2020.101745
issn: 1873-6300
Appears in Collections:(IC) Artículos
Files in This Item:
File Description SizeFormat 
Calbindin immunostaining.pdf3,21 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.