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Title

Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major

AuthorsRastrojo, Alberto ; Corvo, Laura ; Lombraña, Rodrigo ; Solana, José Carlos; Aguado, Begoña ; Requena, José María
Issue Date6-May-2019
PublisherSpringer Nature
CitationScientific Reports 9 (2019)
AbstractBesides their medical relevance, Leishmania is an adequate model for studying post-transcriptional mechanisms of gene expression. In this microorganism, mRNA degradation/stabilization mechanisms together with translational control and post-translational modifications of proteins are the major drivers of gene expression. Leishmania parasites develop as promastigotes in sandflies and as amastigotes in mammalians, and during host transmission, the parasite experiences a sudden temperature increase. Here, changes in the transcriptome of Leishmania major promastigotes after a moderate heat shock were analysed by RNA-seq. Several of the up-regulated transcripts code for heat shock proteins, other for proteins previously reported to be amastigote-specific and many for hypothetical proteins. Many of the transcripts experiencing a decrease in their steady-state levels code for transporters, proteins involved in RNA metabolism or translational factors. In addition, putative long noncoding RNAs were identified among the differentially expressed transcripts. Finally, temperature-dependent changes in the selection of the spliced leader addition sites were inferred from the RNA-seq data, and particular cases were further validated by RT-PCR and Northern blotting. This study provides new insights into the post-transcriptional mechanisms by which Leishmania modulate gene expression.
Publisher version (URL)http://dx.doi.org/10.1038/s41598-019-43354-9
URIhttp://hdl.handle.net/10261/217041
Identifiersdoi: 10.1038/s41598-019-43354-9
issn: 2045-2322
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