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Título: | Identification of Arabidopsis mutants with altered freezing tolerance |
Autor: | Perea-Resa, Carlos CSIC ORCID; Catalá, Rafael CSIC ORCID ; Salinas, Julio CSIC ORCID | Palabras clave: | Freezing temperature Freezing tolerance Cold acclimation Arabidopsis thaliana |
Fecha de publicación: | 1-jul-2020 | Editor: | Humana Press | Citación: | Plant Cold Acclimation. Hincha D., Zuther E. (eds). Methods in Molecular Biology 2156: 85-97 (2020) | Resumen: | Low temperature is an important determinant in the configuration of natural plant communities and defines the range of distribution and growth of important crops. Some plants, including Arabidopsis thaliana, have evolved sophisticated adaptive mechanisms to tolerate freezing temperatures. Central to this adaptation is the process of cold acclimation. By means of this process, many plants from temperate regions are able to develop or increase their freezing tolerance in response to low, nonfreezing temperatures. The identification and characterization of factors involved in freezing tolerance is crucial to understand the molecular mechanisms underlying the cold acclimation response and has a potential interest to improve crop tolerance to freezing temperatures. Many genes implicated in cold acclimation have been identified in numerous plant species by using molecular approaches followed by reverse genetic analysis. Remarkably, however, direct genetic analyses have not been conveniently exploited in their capacity for identifying genes with pivotal roles in that adaptive response. In this chapter, we describe a protocol for evaluating the freezing tolerance of both nonacclimated and cold acclimated Arabidopsis plants. This protocol allows for the accurate and simple screening of mutant collections for the identification of novel factors involved in freezing tolerance and cold acclimation. | Descripción: | 18 p.-3 fig. | Versión del editor: | https://doi.org/10.1007/978-1-0716-0660-5_8 | URI: | http://hdl.handle.net/10261/216789 | DOI: | 10.1007/978-1-0716-0660-5_8 | ISSN: | 978-1-0716-0659-9 |
Aparece en las colecciones: | (CIB) Libros y partes de libros |
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MMB_Perea-Resa_2020.doc | Artículo principal | 91 kB | Microsoft Word | Visualizar/Abrir |
Figures MMB_Perea-Resa_2020 .ppt | Figuras | 624,5 kB | Microsoft Powerpoint | Visualizar/Abrir |
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