English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/216644
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Detection of pathogenic Bacteria by Electrochemical Impedance Spectroscopy: Influence of the immobilization strategies on the sensor performance

AuthorsBarreiros dos Santos, M.; Sporer, C.; Sanvicens, Nuria ; Pascual Duran, Nuria ; Errachid, A.; Martinez, E.; Marco, María Pilar ; Teixeira, V.; Samitier, J.
Issue Date2009
CitationProcedia Chemistry 1: 1291- 1294 (2009)
AbstractElectrochemical impedance spectroscopy (EIS) is applied to detect pathogenic E. coli O157:H7 bacteria via a label free immunoassay-based detection method. Polyclonal anti-E.coli antibodies (PAb) are immobilized onto gold electrodes following two different strategies, via chemical bond formation between antibody amino groups and a carboxylic acid containing self-assembled molecular monolayer (SAM) and alternatively by linking a biotinylated anti-E. coli to Neutravidin on a mixed-SAM. Impedance spectra for sensors of both designs for increasing concentrations of E. coli are recorded in phosphate buffered saline (PBS). The Nyquist plots can be modeled with a Randle equivalent circuit, identifying the charge transfer resistance RCT as the relevant concentration dependent parameter. Sensors fabricated from both designs are able to detect very low concentration of E. coli with limits of detection as low as 10-100 cfu/ml. The influence of the different immobilization protocols on the sensor performance is evaluated in terms of sensitivity, dynamic range and resistance against nonspecific absorption.
Publisher version (URL)http://dx.doi.org/10.1016/j.proche.2009.07.322
URIhttp://hdl.handle.net/10261/216644
DOIhttp://dx.doi.org/10.1016/j.proche.2009.07.322
Identifiersdoi: 10.1016/j.proche.2009.07.322
issn: 1876-6196
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
1-s2.0-S1876619609003234-main.pdf488,83 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.