English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/216459
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Microvirga sp. symbiovar mediterranense nodulates Lupinus cosentinii grown wild in Morocco

AuthorsMissbah El Idrissi, M.; Lamin, H.; ElFaik, S.; Tortosa Muñoz, Germán; Peix, Álvaro ; Bedmar, Eulogio J.; Abdelmoumen, H.
KeywordsAgriculture
Diversity
Microbial phylogenetics
Soil
Symbiosis
Issue Date2020
PublisherBlackwell Publishing
CitationJournal of Applied Microbiology 128: 1109- 1118 (2020)
AbstractAim: To analyse the diversity of nodule-forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results: Of the 31 bacterial strains, four were selected based on their REP-PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii-isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions: To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule-forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study: In this work, we show that Microvirga sp. can be isolated from root nodules of wild-grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.
Publisher version (URL)http://dx.doi.org/10.1111/jam.14526
URIhttp://hdl.handle.net/10261/216459
DOIhttp://dx.doi.org/10.1111/jam.14526
Identifiersdoi: 10.1111/jam.14526
issn: 1365-2672
Appears in Collections:(IRNASA) Artículos
(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.