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Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode

AuthorsGarcía-Molina, Gabriel; Natale, Paolo; Valenzuela, L.; Álvarez-Malmagro, Julia; Gutiérrez Sánchez, Cristina ; Iglesias Juez, Ana ; López-Montero, Iván; Vélez, Marisela ; Pita, Marcos ; López de Lacey, Antonio
Potentiometric biosensor
Biomimetic membrane
Issue Date2020
CitationBioelectrochemistry 133 (2020)
Abstract[EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination.
Publisher version (URL)http://dx.doi.org/10.1016/j.bioelechem.2020.107490
Identifiersdoi: 10.1016/j.bioelechem.2020.107490
e-issn: 1878-562X
issn: 1567-5394
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