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Title

2-D DIGE analysis of Senegalese sole (Solea senegalensis) testis proteome in wild-caught and hormone-treated F1 fish

AuthorsForné, Ignasi; Agulleiro Gozalbo, Maria Josep ; Asensio, Esther; Abián, Joaquín ; Cerdà, Joan
KeywordsAndrogen
Flatfish
GnRHa
Spermatogenesis
Testis
Issue DateApr-2009
PublisherWiley-VCH
CitationProteomics 9(8): 2171-2181 (2009)
AbstractIn the farmed flatfish Senegalese sole, F1 males reared in captivity often show lower sperm production and fertilization capacity than wild-caught males. To gain insights into the molecular mechanismsthatmay be altered in the F1 testis,we used 2-DDIGE tocompare the protein profiling of the testis of wild-caught males at the spermiation stage with that of F1 males showing different stages of germcell development after hormone treatment in vivo. The abundance of 58 out of 1014 protein spots was found to differ significantly between the groups. De novo identification of these proteins byMS/MSrevealed that proteins implicated in oxidoreductase activity, protein catabolism, formation of the zona pellucida receptor, cytoskeleton organization, and lipid binding and metabolism, were regulated in the F1 testes as germcell development progressed. However, distinct isoforms or PTMs of some of these proteins, as well as of proteins involved in iron and glucose metabolism and ATP production, were expressed at lower levels in the testes of F1 males than in wild fish regardless of the hormone treatment. These results contribute to identifying proteins associated with spermatogenesis not previously described in teleosts, and suggest potential mechanisms that may be involved in the poor reproductive performance of Senegalese sole F1 males
Description11 pages, 4 figures, 1 table
Publisher version (URL)http://dx.doi.org/10.1002/pmic.200800696
URI10261/21612
DOI10.1002/pmic.200800696
ISSN1615-9853
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