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Functional characterization of the origin binding domain of the replication initiator RepB: identification of the residues involved in the specific recognition of the cognate origin and in the catalytic activity

AuthorsBordanaba-Ruiseco, Lorena; Ruiz-Masó, José A. ; Solar, Gloria del
KeywordsRolling circle replication
Replication initiator
Protein-DNA binding
Issue DateMay-2013
PublisherFundación General de la Universidad de Alcalá
Instituto de Salud Carlos III (España)
CSIC - Instituto de Química Física Rocasolano (IQFR)
CSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)
CitationXI European Meeting on the Molecular Biology of the Pneumococcus (EuroPneumo 2013)
AbstractSeveral mutants in the origin-binding domain of RepB, the initiator of replication of plasmid pMV158, were constructed and purified. Analysis of these mutants provided information on the structural motifs of RepB involved in the specific recognition of the plasmid origin and on the role of different residues of the catalytic site.
Description1 p.-1 fig.
Publisher version (URL)http://europneumo2013.iqfr.csic.es/EP-2013-Program-and-Abstracts-Book.pdf
Appears in Collections:(CIB) Material de divulgación
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