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| Title: | Functional characterization of the origin binding domain of the replication initiator RepB: identification of the residues involved in the specific recognition of the cognate origin and in the catalytic activity |
| Authors: | Bordanaba-Ruiseco, Lorena; Ruiz-Masó, José A.  ; Solar, Gloria del |
| Keywords: | Rolling circle replication Replication initiator Protein-DNA binding Endonuclease |
| Issue Date: | May-2013 |
| Publisher: | Fundación General de la Universidad de Alcalá Instituto de Salud Carlos III (España) CSIC - Instituto de Química Física Rocasolano (IQFR) CSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB) |
| Citation: | XI European Meeting on the Molecular Biology of the Pneumococcus (EuroPneumo 2013) |
| Abstract: | Several mutants in the origin-binding domain of RepB, the initiator of replication of plasmid pMV158, were constructed and purified. Analysis of these mutants provided information on the structural motifs of RepB involved in the specific recognition of the plasmid origin and on the role of different residues of the catalytic site. |
| Description: | 1 p.-1 fig. |
| Publisher version (URL): | http://europneumo2013.iqfr.csic.es/EP-2013-Program-and-Abstracts-Book.pdf |
| URI: | http://hdl.handle.net/10261/215985 |
| Appears in Collections: | (CIB) Material de divulgación |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| EP-2013-Bordanaba-Ruiseco.pdf | Abstract | 1,36 MB | Adobe PDF |  View/Open |
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