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dc.contributor.authorLemus-Conejo, Anaes_ES
dc.contributor.authorMillán-Linares, María del Carmenes_ES
dc.contributor.authorToscano, Rocíoes_ES
dc.contributor.authorMillán, Franciscoes_ES
dc.contributor.authorPedroche, Justoes_ES
dc.contributor.authorMuriana, Francisco J. G.es_ES
dc.contributor.authorMontserrat-de la Paz, Sergioes_ES
dc.identifier.citationNutritional Neuroscience (2020)es_ES
dc.description.abstractObjectives: Neuroinflammation is a complex inflammatory process in the central nervous system (CNS) where microglia may play a critical role. GPETAFLR is a peptide isolated from Lupinus angustifolius L. protein hydrolysates with functional activity in mononuclear phagocytes. However, it is unknown whether GPETAFLR has neuroprotective effects. Methods: We analysed the potential anti-neuroinflammatory activity of GPETAFLR by using two different models of neuroinflammation: BV-2 microglial cells and mice with high-fat diet (HFD)-induced obesity. Results: GPETAFLR hampered LPS-induced upregulation of pro-inflammatory and M1 marker genes in BV-2 cells. This effect was accompanied by an unchanged expression of anti-inflammatory IL-10 gene and by an increased expression of M2 marker genes. GPETAFLR also increased the transcriptional activity of M2 marker genes, while the microglia population remained unchanged in number and M1/M2 status in brain of mice with high-fat diet (HFD)-induced obesity. Furthermore, GPETAFLR counteracted HFD-induced downregulation of IL-10 and upregulation of pro-inflammatory markers in the mouse brain, both at gene and protein levels. Discussion: This is the first report describing that a peptide from plant origin robustly restrained the pro-inflammatory activation of microglial cells in cultures and in brain. Our data suggest that GPETAFLR might be instrumental in maintaining CNS homeostasis by inhibiting neuroinflammation.es_ES
dc.description.sponsorshipThis work was supported by grants AGL2012-40247-C02 and CYTED -2019 119RT0567 from The Spanish Ministry of Science, Innovation and Universities.es_ES
dc.publisherTaylor & Francises_ES
dc.subjectGPETAFLR peptidees_ES
dc.subjectBV-2 cell linees_ES
dc.subjectHigh-fat dietes_ES
dc.subjectProtein hydrolysateses_ES
dc.subjectLupinus angustifoliuses_ES
dc.titleGPETAFLR, a peptide from Lupinus angustifolius L. prevents inflammation in microglial cells and confers neuroprotection in braines_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
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