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Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria

AuthorsManzoni Maroneze, Mariana; Caballero-Guerrero, Belén ; Queiroz Zepka, Leila; Jacob-Lopes, Eduardo; Pérez Gálvez, Antonio ; Roca, María
Issue Date13-May-2020
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 68 (22): 6212–6220 (2020)
AbstractCyanobacteria and microalgae are characterized by a rich and varied profile of chlorophyll (8–20 mg/g) and carotenoid (>2.2 mg/g) pigments, being noteworthy material for natural pigment production in the food industry. We propose a systematic workflow that uses high-performance liquid chromatography (HPLC) coupled with high-resolution tandem mass spectrometry in a broadband collision-induced dissociation mode (bbCID) acquisition mode to simultaneously obtain MS and MSn spectra. Metabolomic studies showed for the first time the presence of carotenoids with 5,6-epoxy-groups (5,6-epoxy- and 5,8-furanoid β-cryptoxanthin), carotenoids from the α-branch (5,8-furanoid-2′-3′-didehydro α-cryptoxanthin), and 2′-dehydrodeoxomyxol in cyanobacteria. To support the new findings, an in silico search retrieved the putative sequences of carotenogenic enzymes involved in the corresponding biosynthetic pathways (ZEP, NSY, CrtL-b and CrtR) in the analyzed cyanobacteria species. Consequently, high-throughput metabolomics studies assisted by molecular analysis offer a powerful tool for providing insights into the characterization of bioactive compounds and their metabolism in cyanobacteria and microalgae.
Description3 Figuras.-- 3 Tablas
Publisher version (URL)http://dx.doi.org/10.1021/acs.jafc.0c01306
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