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Title: | Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria |
Authors: | Manzoni Maroneze, Mariana; Caballero-Guerrero, Belén CSIC ORCID ; Queiroz Zepka, Leila; Jacob-Lopes, Eduardo; Pérez Gálvez, Antonio CSIC ORCID ; Roca, María CSIC ORCID | Keywords: | Carotenoids α-cryptoxanthin Cyanobacteria 2′-dehydrodeoxomyxol Metabolomics |
Issue Date: | 13-May-2020 | Publisher: | American Chemical Society | Citation: | Journal of Agricultural and Food Chemistry 68 (22): 6212–6220 (2020) | Abstract: | Cyanobacteria and microalgae are characterized by a rich and varied profile of chlorophyll (8–20 mg/g) and carotenoid (>2.2 mg/g) pigments, being noteworthy material for natural pigment production in the food industry. We propose a systematic workflow that uses high-performance liquid chromatography (HPLC) coupled with high-resolution tandem mass spectrometry in a broadband collision-induced dissociation mode (bbCID) acquisition mode to simultaneously obtain MS and MSn spectra. Metabolomic studies showed for the first time the presence of carotenoids with 5,6-epoxy-groups (5,6-epoxy- and 5,8-furanoid β-cryptoxanthin), carotenoids from the α-branch (5,8-furanoid-2′-3′-didehydro α-cryptoxanthin), and 2′-dehydrodeoxomyxol in cyanobacteria. To support the new findings, an in silico search retrieved the putative sequences of carotenogenic enzymes involved in the corresponding biosynthetic pathways (ZEP, NSY, CrtL-b and CrtR) in the analyzed cyanobacteria species. Consequently, high-throughput metabolomics studies assisted by molecular analysis offer a powerful tool for providing insights into the characterization of bioactive compounds and their metabolism in cyanobacteria and microalgae. | Description: | 3 Figuras.-- 3 Tablas | Publisher version (URL): | http://dx.doi.org/10.1021/acs.jafc.0c01306 | URI: | http://hdl.handle.net/10261/215822 | ISSN: | 0021-8561 | E-ISSN: | 1520-5118 |
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