Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/215806
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome |
Autor: | Sentandreu, Enrique CSIC ORCID ; Fuente García, Claudia; Navarro Fabra, José Luis CSIC ORCID; Sentandreu, Miguel Angel CSIC ORCID | Palabras clave: | Proteomics Food proteins Quality assessment Chromatography analysis |
Fecha de publicación: | 29-jun-2020 | Editor: | Sage Publications | Citación: | Food Science and Technology International 27 (2): 112-122 (2021) | Resumen: | Bovine sarcoplasmic sub-proteome was studied through a straightforward gel-free pipeline supported by liquid isoelectric focusing (OFFGEL) protein fractionation coupled to liquid chromatography-mass spectrometry (LC-MS) analysis. Full-MS and data-dependent MS/MS analyses were simultaneously performed by a conventional three-dimensional ion-trap addressing targeted quantitative and untargeted qualitative research, respectively. There were unambiguously identified 47 proteins distributed along 12 OFFGEL fractions assayed. Regarding intermediate- and high-abundant peptides, bulky quantitative data processing performed by MZmine 2 freeware yielded a satisfactory linearity and coefficient of variation with r2 in the 0.95–0.99 range and about 25%, respectively. Up to 41 peptides from 20 identified proteins were relatively quantified throughout OFFGEL fractions. This reliable, flexible and affordable gel-free proteomic approach could be readily implemented by industry to improve quality assessment of protein-based food products. | Versión del editor: | https://doi.org/10.1177/1082013220929144 | URI: | http://hdl.handle.net/10261/215806 | DOI: | 10.1177/1082013220929144 | ISSN: | 1082-0132 |
Aparece en las colecciones: | (IATA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Sentandreu-FSTI2020.pdf | Artículo principal + Material Suplementario | 1,05 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
4
checked on 22-abr-2024
WEB OF SCIENCETM
Citations
3
checked on 22-feb-2024
Page view(s)
130
checked on 24-abr-2024
Download(s)
178
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.