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dc.contributor.authorMenéndez, Esther-
dc.contributor.authorFlores Félix, José David-
dc.contributor.authorRamírez-Bahena, Marta-Helena-
dc.contributor.authorIgual, José Mariano-
dc.contributor.authorGarcía-Fraile, Paula-
dc.contributor.authorPeix, Álvaro-
dc.contributor.authorVelázquez, Encarna-
dc.date.accessioned2020-06-30T16:39:58Z-
dc.date.available2020-06-30T16:39:58Z-
dc.date.issued2020-06-22-
dc.identifier.citationMicroorganisms 8(6): 939 (2020)-
dc.identifier.issn2076-2607-
dc.identifier.urihttp://hdl.handle.net/10261/215689-
dc.description© 2020 by the authors.-
dc.description.abstractIn the present work, we analyse the genomic and phenotypic characteristics of a strain named RZME27T isolated from roots of a Zea mays plant grown in Spain. The phylogenetic analyses of 16S rRNA gene and whole genome sequences showed that the strain RZME27T clustered with the type strains of Neorhizobium galegae and Pseudorhizobium pelagicum from the family Rhizobiaceae. This family encompasses several genera establishing symbiosis with legumes, but the genes involved in nodulation and nitrogen fixation are absent in its genome. Nevertheless, genes related to plant colonization, such as those involved in motility, chemotaxis, quorum sensing, exopolysaccharide biosynthesis and hydrolytic enzymes production were found. The comparative pangenomic analyses showed that 78 protein clusters present in the strain RZME27T were not found in the type strains of its closest relatives N. galegae and P. pelagicum. The calculated average nucleotide identity (ANI) values between the strain RZME27T and the type strains of N. galegae and P. pelagicum were 75.61% and 75.1%, respectively, similar or lower than those found for other genera from family Rhizobiaceae. Several phenotypic differences were also found, highlighting the absence of the fatty acid C19:0 cyclo ω8c and propionate assimilation. These results support the definition of a novel genus and species named Endobacterium cerealis gen. nov. sp. nov. whose type strain is RZME27T.-
dc.description.sponsorshipThis work was supported by MINECO (Spanish Central Government) Grant AGL2013-48098-P to EV. EM was supported by a postdoctoral contract associated to this project and then, with a FCT contract from the Individual Call to Scientific Employment Stimulus 2017 (CEECIND/00270/2017). J.D.F.-F. was the recipient of a predoctoral fellowship from University of Salamanca-
dc.language.isoeng-
dc.publisherMultidisciplinary Digital Publishing Institute-
dc.relationMINECO/ICTI2013-2016/AGL2013-48098-P-
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccess-
dc.subjectEndobacterium gen. nov.-
dc.subjectEndobacterium cerealis sp. nov.-
dc.subjectZea mays-
dc.subjectSpain-
dc.subjectEndophytes-
dc.subjectGenome analysis-
dc.titleGenome Analysis of Endobacterium cerealis, a Novel Genus and Species Isolated from Zea mays Roots in North Spain-
dc.typeartículo-
dc.identifier.doihttp://dx.doi.org/10.3390/microorganisms8060939-
dc.description.peerreviewedPeer reviewed-
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms8060939-
dc.identifier.e-issn2076-2607-
dc.date.updated2020-06-30T16:39:59Z-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderUniversidad de Salamanca-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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