English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/215683
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Exploring the microalga Euglena cantabrica by pressurized liquid extraction to obtain bioactive compounds

AuthorsMuñoz-Almagro, Nerea ; Gilbert-López, Bienvenida; Pozuelo Rollón, María del Carmen; García-Fernandez, Yolanda; Almeida, Carlos; Villamiel, Mar ; Mendiola, J. A. ; Ibáñez, Elena
KeywordsEuglena cantabrica
Pressurized liquid extraction
Response surface
Issue Date12-Jun-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationMarine Drugs 18(6): 308 (2020)
AbstractIn the present study, the chemical composition of the microalga Euglena cantabrica was investigated. The extraction of bioactive compounds was done using pressurized liquid extraction (PLE) at different temperatures (40–180 °C) and using green solvents (ethanol-water mixtures). A statistical design of experiments was used to optimize the maximum antioxidant capacity of the extracts by response surface methodology. The antioxidant capacity was determined through the inhibition of 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, while the chemical analyses of the extracts were carried out using different chromatographic techniques. Chlorophylls and carotenoids were analyzed by high-performance liquid chromatography coupled to a diode array detector and mass spectrometry (HPLC-DAD-MS/MS) and carbohydrates by gas chromatography with flame ionization detection (GC-FID) and high-pressure size-exclusion chromatography coupled to an evaporative light-scattering detector (HPSEC-ELSD). The results showed different possibilities for the extraction conditions, depending on the desired bioactivity or chemical composition. Briefly, (i) mixtures of ethanol-water containing around 40% ethanol at 180 °C gave the best antioxidant capacity, (ii) mixtures containing around 50% ethanol at 110 °C gave the best yield of β-glucan paramylon, and (iii) the use of pure ethanol at a low temperature (40 °C) is the best choice for the recovery of carotenoids such as diatoxanthin. Summing up, E. cantabrica seems to be a good candidate to be used in biorefinery to obtain different bioactive compounds
DescriptionThis article belongs to the Special Issue Innovative Extraction Technology Aiming the Screening and Discovery of Bio-Active Marine Compounds.
Publisher version (URL)https://doi.org/10.3390/md18060308
Appears in Collections:(CIAL) Artículos
Files in This Item:
File Description SizeFormat 
Exploring_Muñoz_Almagro.pdf2,88 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.