English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/215629
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Inoculation with Different Nitrogen-Fixing Bacteria and Arbuscular Mycorrhiza Affects Grain Protein Content and Nodule Bacterial Communities of a Fava Bean Crop

AuthorsSánchez-Navarro, Virginia; Zornoza, Raúl; Faz, Ángel; Egea-Gilabert, Catalina; Ros Muñoz, Margarita Matilde ; Pascual, J. A. ; Fernández, J. A.
KeywordsVicia faba
Rhizobium sp.
Burkholderia sp.
Biological nitrogen fixation
Bacterial community
Issue Date28-May-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationAgronomy 10(6): 768 (2020)
AbstractThe introduction of nitrogen fixing bacteria (NFB) and arbuscular mycorrhizal fungi (AMF) into the soil is an advisable agricultural practice for the crop, since it enhances nutrient and water uptake and tolerance to biotic and abiotic stresses. The aim of this work was to study plant nutrition, biological nitrogen fixation (BNF) and crop yield and quality, after inoculating seeds with NFBs ((Rhizobium leguminosarum, Burkholderia cenocepacia, Burkholderia vietnamiensis)) and/or AMFs (Rhizophagus irregularis, Claroideoglomus etunicatum, Claroideoglomus claroideum and Funneliformis mosseae) in a fava bean crop in two seasons. The composition of the nodule bacterial community was evaluated by the high-throughput sequencing analysis of bacterial 16 S rRNA genes. It was found that microbial inoculation accompanied by a 20% decrease in mineral fertilization had no significant effect on crop yield or the nutritional characteristics compared with a non-inoculated crop, except for an increase in the grain protein content in inoculated plants. None of the inoculation treatments increased biological nitrogen fixation over a non-inoculated level. The bacterial rRNA analysis demonstrated that the genus Rhizobium predominated in all nodules, both in inoculated and non-inoculated treatments, suggesting the previous presence of these bacteria in the soil. In our study, inoculation with Rhizobium leguminosarum was the most effective treatment for increasing protein content in seeds, while Burkholderia sp. was not able to colonise the plant nodules. Inoculation techniques used in fava beans can be considered an environmentally friendly alternative, reducing the input of fertilizers, while maintaining crop yield and quality, with the additional benefit of increasing the grain protein content. However, further research is required on the selection and detection of efficient rhizobial strains under local field conditions, above all those related to pH and soil type, in order to achieve superior nitrogen-fixing bacteria.
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/agronomy10060768
URIhttp://hdl.handle.net/10261/215629
DOIhttp://dx.doi.org/10.3390/agronomy10060768
E-ISSN2073-4395
Appears in Collections:(CEBAS) Artículos
Files in This Item:
File Description SizeFormat 
Inoculation_Sanchez_Navarro_Art2020.pdf893,6 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.