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Title

Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells

AuthorsPablo, Lorena de ; Echeverría, Irache; Rius-Rocabert, Sergio; Luján, Lluís; Garcin, Dominique; Andrés, Damián F. de ; Nistal-Villán, Estanislao; Reina, Ramsés
KeywordsSmall ruminant lentivirus
Sendai virus
Innate immunity
Interferon
APOBEC3
Issue Date2020
PublisherMultidisciplinary Digital Publishing Institute
CitationVaccines 8(2): 206 (2020)
AbstractSmall ruminant lentiviruses (SRLVs) are widely spread in the ovine and caprine populations, causing an incurable disease affecting animal health and production. Vaccine development is hindered owing to the high genetic heterogeneity of lentiviruses and the selection of T-cell and antibody escape mutants, requiring antigen delivery optimization. Sendai virus (SeV) is a respiratory paramyxovirus in mice that has been recognized as a potent inducer of innate immune responses in several species, including mouse and human. The aim of this study was to stimulate an innate antiviral response in ovine cells and evaluate the potential inhibitory effect upon small ruminant lentivirus (SRLV) infections. Ovine alveolar macrophages (AMs), blood-derived macrophages (BDMs), and skin fibroblasts (OSFs) were stimulated through infection with SeV encoding green fluorescent protein (GFP). SeV efficiently infected ovine cells, inducing an antiviral state in AM from SRLV naturally-infected animals, as well as in in vitro SRLV-infected BDM and OSF from non-infected animals. Supernatants from SeV-infected AM induced an antiviral state when transferred to fresh cells challenged with SRLV. Similar to SRLV, infectivity of an HIV-1-GFP lentiviral vector was also restricted in ovine cells infected with SeV. In myeloid cells, an M1-like proinflammatory polarization was observed together with an APOBEC3Z1 induction, among other lentiviral restriction factors. Our observations may boost new approximations in ameliorating the SRLV burden by stimulation of the innate immune response using SeV-based vaccine vectors.
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/vaccines8020206
URIhttp://hdl.handle.net/10261/215624
DOI10.3390/vaccines8020206
ISSN2076-393X
E-ISSN2076-393X
Appears in Collections:(IDAB) Artículos
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