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Title

On the Importance of the Thiazole Nitrogen in Epothilones: Semisynthesis and Microtubule-Binding Affinity of Deaza-Epothilone C

AuthorsEdenharter, Adriana; Ryckewaert, Lucie; Cintulová, Daniela; Estévez-Gallego, Juan; Díaz, José Fernando ; Altmann, Karl-Heinz
KeywordsBinding affinity
Epothilones
Deaza-epothilone
Microtubules
Structure-activity relationship
Thiophene
Issue Date2020
PublisherMultidisciplinary Digital Publishing Institute
CitationChemistry 2(2): 499-509 (2020)
AbstractDeaza-epothilone C, which incorporates a thiophene moiety in place of the thiazole heterocycle in the natural epothilone side chain, has been prepared by semisynthesis from epothilone A, in order to assess the contribution of the thiazole nitrogen to microtubule binding. The synthesis was based on the esterification of a known epothilone A-derived carboxylic acid fragment and a fully synthetic alcohol building block incorporating the modified side chain segment and subsequent ring-closure by ring-closing olefin metathesis. The latter proceeded with unfavorable selectivity and in low yield. Distinct differences in chemical behavior were unveiled between the thiophene-derived advanced intermediates and what has been reported for the corresponding thiazole-based congeners. Compared to natural epothilone C, the free energy of binding of deaza-epothilone C to microtubules was reduced by ca. 1 kcal/mol or less, thus indicating a distinct but non-decisive role of the thiazole nitrogen in the interaction of epothilones with the tubulin/microtubule system. In contrast to natural epothilone C, deaza-epothilone C was devoid of antiproliferative activity in vitro up to a concentration of 10 μM, presumably due to an insufficient stability in the cell culture medium.
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/chemistry2020030
URIhttp://hdl.handle.net/10261/215619
DOI10.3390/chemistry2020030
ISSN0947-6539
E-ISSN1521-3765
Appears in Collections:(CIB) Artículos
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