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dc.contributor.authorConlon, J. M.es_ES
dc.contributor.authorAttoub, Samires_ES
dc.contributor.authorMusale, Vishales_ES
dc.contributor.authorLeprince, Jérômees_ES
dc.contributor.authorCasewell, Nicholas R.es_ES
dc.contributor.authorSanz, Libiaes_ES
dc.contributor.authorCalvete, Juan J.es_ES
dc.date.accessioned2020-06-25T07:58:11Z-
dc.date.available2020-06-25T07:58:11Z-
dc.date.issued2020-03-18-
dc.identifier.citationToxicon: X 6:100030 (2020)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/215201-
dc.description8 páginas, 4 figuras, 1 tablaes_ES
dc.description.abstractFour peptides with cytotoxic activity against BRIN-BD11 rat clonal β-cells were purified from the venom of the black-necked spitting cobra Naja nigricollis using reversed-phase HPLC. The peptides were identified as members of the three-finger superfamily of snake toxins by ESI-MS/MS sequencing of tryptic peptides. The most potent peptide (cytotoxin-1N) showed strong cytotoxic activity against three human tumor-derived cell lines (LC50 = 0.8 ± 0.2 μM for A549 non-small cell lung adenocarcinoma cells; LC50 = 7 ± 1 μM for MDA-MB-231 breast adenocarcinoma cells; and LC50 = 9 ± 1 μM for HT-29 colorectal adenocarcinoma cells). However, all the peptides were to varying degrees cytotoxic against HUVEC human umbilical vein endothelial cells (LC50 in the range 2-22 μM) and cytotoxin-2N was moderately hemolytic (LC50 = 45 ± 3 μM against mouse erythrocytes). The lack of differential activity against cells derived from non-neoplastic tissue limits their potential for development into anti-cancer agents. In addition, two proteins in the venom, identified as isoforms of phospholipase A2, effectively stimulated insulin release from BRIN-BD11 cells (an approximately 6-fold increase in rate compared with 5.6 mM glucose alone) at a concentration (1 μM) that was not cytotoxic to the cells suggesting possible application in therapy for Type 2 diabetes.es_ES
dc.description.sponsorshipThis work was partly supported by the Ministerio de Ciencia, Innovacion � y Universidades, Madrid, Spain (grant number BFU 2017- 89103-P) to JJC.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMICIU/ICTI2017-2020/BFU2017-89103-Pes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectCytotoxicityes_ES
dc.subjectInsulinotropic activityes_ES
dc.subjectNajaes_ES
dc.subjectPhospholipase A2es_ES
dc.subjectThree-finger toxinses_ES
dc.titleIsolation and characterization of cytotoxic and insulin-releasing components from the venom of the black-necked spitting cobra Naja nigricollis (Elapidae)es_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.toxcx.2020.100030-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.toxcx.2020.100030es_ES
dc.identifier.e-issn2590-1710-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
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