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Title

Pathogenesis of Salmonella typhimurium mutants in two experimental models in vivo Patogenia de mutantes de Salmonella typhimurium en dos modelos experimentales in vivo

AuthorsVelasco-Carrillo, Judith; Araque-Granadillo, María; Ayala-Serrano, Juan; Dávila-Vera, Delsy; Peña-Contreras, Zulma; Mendoza-Briceño, Rosa
KeywordsSalmonella
Islas de patogenicidad
Mutación
Microscopía electrónica de transmisión
Ultraestrutur
Issue Date2019
PublisherFinlay Ediciones
CitationVacciMonitor 28: 1- 8 (2019)
AbstractWith the aim of evaluate the pathogenesis in Salmonella Typhimurium strains with mutations in genes invG/invE of Salmonella Pathogenicity Island 1 (SPI-1) and genes ssaJ/ssaK in the SPI-2 models were evaluated ligated intestinal loop associated mouse tissues by observation by transmission electron microscopy (TEM) and the production of mouse systemic salmonellosis. For this, we used six Salmonella strains: S. Typhimurium SL-1344 (control strain) and its derived mutants: ΔinvEG S. Typhimurium SSalmonella, islas de patogenicidad, mutación, microscopía electrónica de transmisión, ultraestruturL-1344 (mutant in SPI-1) and ΔssaJK S. Typhimurium SL-1344 (mutant in SPI-2), S. Typhimurium (clinical isolate) and its derived mutants: ΔinvEG S. Typhimurium and ΔssaJK S. Typhimurium. TEM results allowed us to verify the morphological alterations of the intestinal epithelium in mice infected with Salmonella strains whose pathogenicity genes were intact. It was proven invasive power loss only in strains mutated in the SPI-1. Through systemic salmonellosis model mouse we noted the loss of the ability to spread in both mutants. In conclusion, the models allowed us to verify the importance of the invG/invE genes of SPI-1 and ssaJ/ssaK of SPI-2 in the pathogenesis of salmonellosis, using BALB/c mice as an experimental model of infection. These in vivo models are suggested to evaluate mutants of genes involved in the pathogenesis of Salmonella, since they represent an important tool for the understanding of the Salmonella-host interaction.
URIhttp://hdl.handle.net/10261/215132
Identifiersissn: 1025-0298
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