English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/215132
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Pathogenesis of Salmonella typhimurium mutants in two experimental models in vivo Patogenia de mutantes de Salmonella typhimurium en dos modelos experimentales in vivo

AuthorsVelasco-Carrillo, Judith; Araque-Granadillo, María; Ayala-Serrano, Juan; Dávila-Vera, Delsy; Peña-Contreras, Zulma; Mendoza-Briceño, Rosa
Islas de patogenicidad
Microscopía electrónica de transmisión
Issue Date2019
PublisherFinlay Ediciones
CitationVacciMonitor 28: 1- 8 (2019)
AbstractWith the aim of evaluate the pathogenesis in Salmonella Typhimurium strains with mutations in genes invG/invE of Salmonella Pathogenicity Island 1 (SPI-1) and genes ssaJ/ssaK in the SPI-2 models were evaluated ligated intestinal loop associated mouse tissues by observation by transmission electron microscopy (TEM) and the production of mouse systemic salmonellosis. For this, we used six Salmonella strains: S. Typhimurium SL-1344 (control strain) and its derived mutants: ΔinvEG S. Typhimurium SSalmonella, islas de patogenicidad, mutación, microscopía electrónica de transmisión, ultraestruturL-1344 (mutant in SPI-1) and ΔssaJK S. Typhimurium SL-1344 (mutant in SPI-2), S. Typhimurium (clinical isolate) and its derived mutants: ΔinvEG S. Typhimurium and ΔssaJK S. Typhimurium. TEM results allowed us to verify the morphological alterations of the intestinal epithelium in mice infected with Salmonella strains whose pathogenicity genes were intact. It was proven invasive power loss only in strains mutated in the SPI-1. Through systemic salmonellosis model mouse we noted the loss of the ability to spread in both mutants. In conclusion, the models allowed us to verify the importance of the invG/invE genes of SPI-1 and ssaJ/ssaK of SPI-2 in the pathogenesis of salmonellosis, using BALB/c mice as an experimental model of infection. These in vivo models are suggested to evaluate mutants of genes involved in the pathogenesis of Salmonella, since they represent an important tool for the understanding of the Salmonella-host interaction.
Identifiersissn: 1025-0298
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
Ayala-SerranoJ_PatogeniadeMutantesdeSalmonella.pdf570,03 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.