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dc.contributor.authorDomenech, Mirianes_ES
dc.contributor.authorRuiz, Susanaes_ES
dc.contributor.authorMoscoso, Miriames_ES
dc.contributor.authorGarcía, Ernestoes_ES
dc.date.accessioned2020-06-18T09:37:22Z-
dc.date.available2020-06-18T09:37:22Z-
dc.date.issued2015-10-
dc.identifier.citationEnviron Microbiol Rep 7(5) 715-27 (2015)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/214725-
dc.description13 p.-6 fig.es_ES
dc.description.abstractExtracellular deoxyribonucleic acid (eDNA) is an essential component of bacterial biofilm matrices, and is required in their formation and maintenance. Extracellular DNA binds to exopolysaccharides or extracellular proteins, affording biofilms greater structural integrity. Recently, we reported evidence of intercellular eDNA-LytC complexes in pneumococcal biofilms. The LytC lysozyme is a member of the choline-binding family of proteins (CBPs) located on the pneumococcal surface. The present work shows that other CBPs, i.e. LytA, LytB, Pce, PspC and CbpF, which have a pI between 5 and 6, can bind DNA in vitro. This process requires the presence of divalent cations other than Mg(2+). This DNA binding capacity of CBPs appears to be independent of their enzymatic activity and, at least in the case of LytA, does not require the choline-binding domain characteristic of CBPs. Positively charged, surface-exposed, 25 amino acid-long peptides derived from the catalytic domain of LytB, were also found capable of DNA binding through electrostatic interactions. Confocal laser scanning microcopy revealed the existence of cell-associated LytB-eDNA complexes in Streptococcus pneumoniae biofilms. These and other findings suggest that these surface-located proteins of S. pneumoniae could play roles of varying importance in the colonization and/or invasion of human host where different environmental conditions exist.es_ES
dc.description.sponsorshipThis work was supported by a grant from the Ministerio de Economía y Competitividad (SAF2012-39444-C02-01/02).es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.rightsclosedAccesses_ES
dc.subjectPseudomonas-aeruginosa biofilmses_ES
dc.subjectExtracellular dnaes_ES
dc.subjectNasopharyngeal colonizationes_ES
dc.subjectPhosphorylcholine esterasees_ES
dc.subjectTransition-metalses_ES
dc.subjectCrystal-structurees_ES
dc.subjectEpithelial-cellses_ES
dc.subjectNormal growthes_ES
dc.subjectFactor-hes_ES
dc.subjectMatrixes_ES
dc.titleIn vitro biofilm development of Streptococcus pneumoniae and formation of choline-binding protein–DNA complexeses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/1758-2229.12295-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1111/1758-2229.12295es_ES
dc.identifier.e-issn1758-2229-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.contributor.orcidDomenech, Mirian [0000-0002-0942-8180]es_ES
dc.contributor.orcidMoscoso, Miriam [0000-0001-8977-3269]es_ES
dc.contributor.orcidGarcía, Ernesto [0000-0002-1741-5486]es_ES
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