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Título: | Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs |
Autor: | Pérez-Núñez, Daniel CSIC ORCID; Sunwoo, Sun-Young; Sánchez, Elena G. CSIC; Haley, Nicholas; García-Belmonte, Raquel; Nogal, Marisa; Morozov, Igor; Madden, Daniel; Gaudreault, Natasha N.; Mur, Lina; Shivana, Vinay; Richt, Jüergen A.; Revilla Novella, Yolanda | Palabras clave: | African swine fever virus Heterologous DNA-protein immunization Plasmid-expressed antigen Recombinant protein Immune response Immunopathology |
Fecha de publicación: | 1-nov-2018 | Citación: | Veterinary immunology and immunopathology 208: 34- 43 (2019) | Resumen: | African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed. | Versión del editor: | http://dx.doi.org/10.1016/j.vetimm.2018.11.018 | URI: | http://hdl.handle.net/10261/214533 | DOI: | 10.1016/j.vetimm.2018.11.018 | Identificadores: | doi: 10.1016/j.vetimm.2018.11.018 issn: 1873-2534 |
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