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Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs

AuthorsPérez-Núñez, Daniel ; Sunwoo, Sun-Young; Sánchez, Elena G. ; Haley, Nicholas; García-Belmonte, Raquel; Nogal, Marisa; Morozov, Igor; Madden, Daniel; Gaudreault, Natasha N.; Mur, Lina; Shivana, Vinay; Richt, Jüergen A.; Revilla, Yolanda
KeywordsAfrican swine fever virus
Heterologous DNA-protein immunization
Plasmid-expressed antigen
Recombinant protein
Immune response
Issue Date1-Nov-2018
CitationVeterinary immunology and immunopathology 208: 34- 43 (2019)
AbstractAfrican swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
Publisher version (URL)http://dx.doi.org/10.1016/j.vetimm.2018.11.018
Identifiersdoi: 10.1016/j.vetimm.2018.11.018
issn: 1873-2534
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