Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles

AuthorsMitsuwan, Watcharapong; Jiménez-Munguía, Irene; Visutthi, Monton; Sianglum, Wipawadee; Jover, A.; Barcenilla, F.; García, M. CSIC ORCID; Pujol, Miquel; Gasch, Oriol; Domínguez, María Ángeles; Camoez, Mariana; Dueñas, Carlos; Ojeda, E.; Martínez, José Antonio; Marco, Francesc; Chaves, F.; Lagarde, M.; López-Medrano, Francisco; Montejo, J. M.; Bereciartúa, Elena; Hernández, J. L.; von Wichman, Miguel A.; Goenaga, A.; García-Arenzana, J. M.; Padilla, Belén; Padilla, C.; Cercenado, Emilia; García-Prado, G.; Tapiol. J.; Horcajada, Juan Pablo; Montero, M.; Salvadó, M.; Arnaiz, A.; Fernández, C.; Calbo, Esther; Xercavins, M.; Granados, A.; Fontanals-Aymerich, Dionisi; Pintado, Vicente; Loza, Elena; Torre-Cisneros, Julián; Lara, Rosario; Rodríguez-López, Fernando; Natera, Clara; Blanco, José Ramón; Olarte, I.; Benito, Natividad; Mirelis, B.; Murillas, J.; Ruiz de Gopegui Bordes, Enrique; Espejo, H.; Morera, M. A.; Rodríguez-Baño, Jesús; López-Cortés, Luis Eduardo; Pascual, Álvaro; Martin, C.; Lepe, José A.; Molina Gil-Bermejo, José; Sordé, R.; Almirante, Benito; Larrosa, M. N.
Staphylococcus aureus
Membrane vesicles
Haemolytic activity
Issue DateMar-2019
CitationMicrobial Pathogenesis 128: 112-118 (2019)
AbstractSigma factor B (SigB) controls the expression of Staphylococcus aureus genes including virulence factors and plays a role in the bacterial secretion system through membrane vesicle production. Inhibition of SigB could attenuate SigB dependent virulence and secretion system. The objective of this study was to determine the effects of rhodomyrtone on SigB and virulence factors related to SigB. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of rhodomyrtone against 67 clinical methicillin-resistant S. aureus isolates were 0.25–8 μg/ml, which were similar to those of vancomycin. Using luciferase gene fused to SigB dependent promoters of asp23, five time reduction in SigB activity was observed when the bacteria were treated with rhodomyrtone for 3 h. Rhodomyrtone significantly reduced SigB activity in a concentration dependent manner in exponentially growing cells (P < 0.05). In addition, sigB mutant was more sensitive towards increasing concentrations of rhodomyrtone than the wild type and yabJ-spoVG mutant. Rhodomyrtone at 0.625 μg/ml reduced the growth of sigB mutant by approximately 99%, compared with the yabJ-spoVG mutant and the wild type. Membrane vesicles were significantly reduced in the bacterial cells when treated with 0.5 × MIC rhodomyrtone (P < 0.05). Decreased haemolytic activity was detected within rhodomyrtone-treated membrane vesicles. The results indicated that rhodomyrtone inhibited S. aureus SigB activity during exponentially growing phase and inhibited haemolytic activity within membrane vesicles.
DescriptionREIPI/GEIH Study Group.
Publisher version (URL)
Identifiersdoi: 10.1016/j.micpath.2018.12.019
issn: 0882-4010
Appears in Collections:(IBIS) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work


checked on Jan 21, 2022


checked on Jan 17, 2022

Page view(s)

checked on Jan 22, 2022


checked on Jan 22, 2022

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.