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dc.contributor.authorPicard-Sánchez, Amparoes_ES
dc.contributor.authorPiazzon de Haro, María Carlaes_ES
dc.contributor.authorAhmed, Nahla Hossameldines_ES
dc.contributor.authorPozo, R. deles_ES
dc.contributor.authorSitjà-Bobadilla, Ariadnaes_ES
dc.contributor.authorPalenzuela, Oswaldoes_ES
dc.date.accessioned2020-06-15T06:42:25Z-
dc.date.available2020-06-15T06:42:25Z-
dc.date.issued2020-06-12-
dc.identifier.citationVeterinary Pathology 57(4): 565-576 (2020)es_ES
dc.identifier.issn0300-9858-
dc.identifier.urihttp://hdl.handle.net/10261/214285-
dc.description.abstractEnterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream (Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hinder its research. This study investigated the associated lesions, its detection by quantitative polymerase chain reaction, and the cellular immune response of naturally infected fish. The intensity of infection in the intestine was correlated with stunted growth and reduced body condition. At the beginning of the outbreaks, infection prevalence was highest in intestine and stomach, and in subsequent months, the prevalence decreased in the intestine and increased in hematopoietic organs and stomach. In heavy infections, the intestine had histologic lesions of enterocyte hypercellularity and proliferation of rodlet cells. Infected enterocytes had E. nucleophila spores in the cytoplasm, and a pyknotic nucleus, karyorhexis or karyolysis. Lymphocytes were present at the base of the mucosa, and eosinophilic granule cells were located between the enterocytes. In intestinal submucosa, macrophage aggregates containing spores were surrounded by lymphocytes and granulocytes, with submucosal infiltration of granulocytes. Macrophage aggregates appeared to develop into granulomata with necrotic areas containing parasite remnants. Immunohistochemistry revealed mast cells as the main type of granulocyte involved. Abundant IgM+ and IgT+ cells were identified by in situ hybridization in the submucosa when intracytoplasmic stages were present. This study describes the lesions of E. nucleophila in gilthead sea bream, an important aquaculture species.es_ES
dc.description.sponsorshipThis work has been carried out with financial support from the European Union and the Spanish MINECO under grant projects ParaFishControl (H2020-634429) and AGL2013-R-48560-C2-2-R, respectively. APS was contracted under ParaFishControl project, RDP was contracted under the PTA-Program from the Spanish Ministry of Science, Innovation and Universities (PTA2018-015315-I), and MCP under CSIC PIE Project No. 201740E013. NHA was the recipient of a fellowship from the Egyptian Cultural Office in Spain.es_ES
dc.language.isoenges_ES
dc.publisherSage Publicationses_ES
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/634429es_ES
dc.relationMINECO/ICTI2013-2016/AGL2013-R-48560-C2-2-Res_ES
dc.relationMICIU/ICTI2017-2020/PTA2018-015315-Ies_ES
dc.relationPTA2018-015315-I/AEI/10.13039/501100011033es_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectEnterocytozoonidaees_ES
dc.subjectHistopathologyes_ES
dc.subjectImmunoglobulinses_ES
dc.subjectGranulocyteses_ES
dc.subjectGrowth retardationes_ES
dc.subjectqPCRes_ES
dc.subjectFishes_ES
dc.titleEnterospora nucleophila (Microsporidia) in Gilthead Sea Bream (Sparus aurata): Pathological Effects and Cellular Immune Response in Natural Infectionses_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1177/0300985820927707-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1177/0300985820927707es_ES
dc.identifier.e-issn1544-2217-
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
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