English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/214199
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp

AuthorsTuttobene, Marisel Romina; Fernández-García, Laura; Blasco, Lucía; Cribb, Pamela; Ambroa, Antón; Müller, Gabriela Leticia; Fernández-Cuenca, Felipe; Bleriot, Inés; Rodríguez, Ramiro Esteban; Barbosa, Beatriz G. V.; López-Rojas, Rafael; Trastoy, Rocío; López, María; Bou, Germán; Tomás, María; Mussi, María A.
KeywordsAcetoin
BLSA
AcoN
Light
Acinetobacter
Issue DateJun-2019
PublisherFrontiers Media
CitationFrontiers in Microbiology 10: 1376 (2019)
AbstractAcinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.
DescriptionCopyright © 2019 Tuttobene, Fernández-García, Blasco, Cribb, Ambroa, Müller, Fernández-Cuenca, Bleriot, Rodríguez, Barbosa, Lopez-Rojas, Trastoy, López, Bou, Tomás and Mussi.
Publisher version (URL)http://dx.doi.org/10.3389/fmicb.2019.01376
URIhttp://hdl.handle.net/10261/214199
DOIhttp://dx.doi.org/10.3389/fmicb.2019.01376
E-ISSN1664-302X
Appears in Collections:(IBIS) Artículos
Files in This Item:
File Description SizeFormat 
Quorum_Tuttobene_Art2019.pdf9,36 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.