English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/214065
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Bacillus Subtilis DisA Regulates RecA-mediated DNA Strand Exchange

AuthorsCarrasco, Begoña; Gándara, Carolina; Baidya, Amit K.; Ben-Yehuda, S.; Alonso, Juan Carlos
AdvisorTorres, Rubén
KeywordsDNA repair
c-di-AMP
RecA nucleation
SsbA
RecO
AddAB
RecJ: DisA
Issue Date4-Jun-2019
PublisherOxford University Press
CitationNucleic Acids Research 47(1): 5141–5154 (2019)
AbstractBacillus subtilis diadenylate cyclase DisA converts two ATPs into c-di-AMP, but this activity is suppressed upon interaction with sites of DNA damage. DisA forms a rapid moving focus that pauses upon induction of DNA damage during spore development. We report that DisA pausing, however, was not observed in the absence of the RecO mediator or of the RecA recombinase, suggesting that DisA binds to recombination intermediates formed by RecA in concert with RecO. DisA, which physically interacts with RecA, was found to reduce its ATPase activity without competing for nucleotides or ssDNA. Furthermore, increasing DisA concentrations inhibit RecA-mediated DNA strand exchange, but this inhibition failed to occur when RecA was added prior to DisA, and was independent of RecA-mediated nucleotide hydrolysis or increasing concentrations of c-di-AMP. We propose that DisA may preserve genome integrity by downregulating RecA activities at several steps of the DNA damage tolerance pathway, allowing time for the repair machineries to restore genome stability. DisA might reduce RecA-mediated template switching by binding to a stalled or reversed fork.
Publisher version (URL)http://doi.org/10.1093/nar/gkz219
URIhttp://hdl.handle.net/10261/214065
DOI10.1093/nar/gkz219
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
Torres et al.pdf4,57 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.