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Title

The interaction of ω2 with the RNA polymerase β′ subunit functions as an activation to repression switch

AuthorsVolante, Andrea; Carrasco, Begoña; Tabone, Mariangela; Alonso, Juan Carlos CSIC ORCID
Issue Date2015
PublisherOxford University Press
CitationNucleic Acids Research 43(19): 9249- 9261 (2015)
AbstractThe ω gene is encoded in broad-host range and low-copy plasmids. It is genetically linked to antibiotic resistance genes of the major human pathogens of phylum Firmicutes. The homodimeric forms of ω (ω) coordinate the plasmid copy number control, faithful partition (ω and δ) and better-than-random segregation (ζεζ) systems. The promoter (P) of the ωεζ operon (P) transiently interacts with ω. Adding δ facilitates the formation of stable ω·P complexes. Here we show that limiting ω interacts with the N-terminal domain of the β′ subunit of the Bacillus subtilis RNA polymerase (RNAP-σ) vegetative holoenzyme. In this way ω recruits RNAP-σ onto P DNA. Partial P occupancy by ω increases the rate at which RNAP-σ complex shifts from its closed (RP) to open (RP) form. This shift increases transcription activation. Adding δ further increases the rate of P transcription initiation, perhaps by stabilizing the ω·P complex. In contrast, full operator occupancy by ω facilitates RP formation, but it blocks RP isomerization and represses P utilization. The stimulation and inhibition of RP formation is the mechanism whereby ω mediates copy number fluctuation and stable plasmid segregation. By this mechanism, ω also indirectly influences the acquisition of antibiotic resistance genes.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkv788
URIhttp://hdl.handle.net/10261/214044
DOI10.1093/nar/gkv788
Identifiersdoi: 10.1093/nar/gkv788
e-issn: 1362-4962
pmid: 26243774
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