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The benefits of carbon black, gold and magnetic nanomaterials for point-of-harvest electrochemical quantification of domoic acid

AuthorsNelis, Joost L.D.; Migliorelli, Davide; Jafari, Safiye; Generelli, Silvia; Lou-Franco, Javier; Salvador, Juan Pablo ; Marco, María Pilar ; Cao, Cuong; Elliott, Christopher T.; Campbell, Katrina
Marine toxin
Food analyses
Material characterization
Point of care
Point of site
Issue Date12-Feb-2020
PublisherSpringer Nature
CitationMicrochimica Acta 187: 164 (2020)
AbstractGold nanostars (GNST), gold nanospheres (GNP) and carbon black (CB) are chosen as alternative nanomaterials to modify carbon screen-printed electrodes (c-SPEs). The resulting three kinds of modified c-SPEs (GNP-SPE, CB-SPE and GNSP-SPE) were electrochemically and microscopically characterized and compared with standardized c-SPEs after pretreatment with phosphate buffer by pre-anodization (pre-SPE). The results show outstanding electrochemical performance of the carbon black-modified SPEs which show low transient current, low capacitance and good porosity. A competitive chronoamperometric immunoassay for the shellfish toxin domoic acid (DA) is described. The performances of the CB-SPE, GNP-SPE and pre-SPE were compared. Hapten-functionalized magnetic beads were used to avoid individual c-SPE functionalization with antibody while enhancing the signal by creating optimum surface proximity for electron transfer reactions. This comparison shows that the CB-SPE biosensor operated best at a potential near − 50 mV (vs. Ag/AgCl) and enables DA to be determined with a detection limit that is tenfold lower compared to pre-SPE (4 vs. 0.4 ng mL−1). These results show very good agreement with HPLC data when analysing contaminated scallops, and the LOD is 0.7 mg DA kg−1 of shellfish.
Publisher version (URL)https://doi.org/10.1007/s00604-020-4150-x
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