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Molecular Mechanisms Controlling the Disease Cycle in the Vascular Pathogen Verticillium dahliae Characterized Through Forward Genetics and Transcriptomics

AuthorsSarmiento Villamil, J.L.; García Pedrajas, N.E.; Cañizares, M.C.; García Pedrajas, M.D.
KeywordsFlbA-like protein
gene deletion method
hemibiotrophic fungus
Verticillium wilt
Issue Date1-Jun-2020
PublisherAmerican Phytopathological Society
CitationMPMI - Molecular Plant Microbe Interactions
AbstractThe soil-borne pathogen Verticillium dahliae has a worldwide distribution and a plethora of hosts of agronomic value. Molecular analysis of virulence processes can identify targets for disease control. In this work, we compared the global gene transcription profile of random T-DNA insertion mutant strain D-10-8F, which exhibits reduced virulence and alterations in microsclerotium formation and polar growth, with that of the wild-type strain. Three genes identified as differentially expressed were selected for functional characterization. To produce deletion mutants, we developed an updated version of one-step construction of Agrobacterium-recombination-ready plasmids (OSCAR) that included the negative selection marker HSVtk (herpes simplex virus thymidine kinase gene) to prevent ectopic integration of the deletion constructs.
Descriptionco-funded by the European Union (European Regional Development Fund)
Publisher version (URL)10.1094/MPMI-08-19-0228-R
Appears in Collections:(IHSM) Artículos
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