Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/213822
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies |
Autor: | Marrero, Patricia CSIC ORCID ; Fregel, Rosa; Cabrera, Vicente M.; Nogales, Manuel CSIC ORCID | Palabras clave: | DNA extraction Ecological studies Guanidine thiocyanate Non-invasive methods Pigeons Seed dispersal |
Fecha de publicación: | 2009 | Editor: | BioMed Central | Citación: | Biological Research 42(2): 147-151 (2009) | Resumen: | DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invasive samples are potential problems for subsequent PCR amplifications. In this study we describe a novel technique for extracting DNA from alimentary tract cells found on external surfaces of feces and regurgitated seeds. The presence of contaminants and inhibitors is minimized and samples are preserved intact for use in other ecological research (e.g. trophic studies). The amplification efficiency and purity of the extracted DNA from feces were significantly higher than in commonly used extraction procedures. Moreover, DNA of two bird species was identified from seeds expelled by regurgitation. Therefore, this method may be suitable for future ecological studies of birds, and other vertebrate groups. | Versión del editor: | http://dx.doi.org/10.4067/S0716-97602009000200002 | URI: | http://hdl.handle.net/10261/213822 | DOI: | 10.4067/S0716-97602009000200002 | ISSN: | 0716-9760 | E-ISSN: | 0717-6287 |
Aparece en las colecciones: | (IPNA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
BR-2009-42-147.pdf | Artículo principal | 87,6 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
28
checked on 12-abr-2024
Page view(s)
136
checked on 16-abr-2024
Download(s)
114
checked on 16-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons