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dc.contributor.authorCastillo, Juan-
dc.contributor.authorCrespo, Diego-
dc.contributor.authorCapilla, Encarnación-
dc.contributor.authorDíaz, Mònica-
dc.contributor.authorChauvigné, François-
dc.contributor.authorCerdà, Joan-
dc.contributor.authorPlanas, Josep V.-
dc.identifier.citationThe American Journal of Physiology- Regulatory Integrative Comparative Physiology 297(5):1570-1581 (2009)en_US
dc.description12 pages, 9 figures, 2 tablesen_US
dc.description.abstractEvolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafish. Am J Physiol Regul Integr Comp Physiol 297: R1570–R1581, 2009. First published September 23, 2009; doi:10.1152/ajpregu.00430.2009.—In mammals, GLUT2 plays an essential role in glucose homeostasis. From an evolutionary perspective, relatively little is known about the biology of GLUT2, or other GLUTs, in nonmammalian vertebrates. Here, we have conducted studies to functionally characterize GLUT2 in zebrafish. First, we cloned the zebrafish ortholog of GLUT2 (zfGLUT2) encoding a protein of 504 amino acids with high-sequence identity to other known vertebrate GLUT2 proteins. The zfGLUT2 gene consists of 11 exons and 10 introns, spanning 20 kb and mapping to a region of chromosome 2 that exhibits conserved synteny with human chromosome 3. When expressed in Xenopus oocytes, zfGLUT2 transported 2-deoxyglucose (2-DG) with similar affinity than mammalian GLUT2 (Km of 11 mM). Transport of 2-DG was competed mostly by D-fructose and D-mannose and was inhibited by cytochalasin B. During early development, zfGLUT2 expression was detected already at 10 h postfertilization and remained elevated in 5-day larvae, when it was clearly localized to the liver and intestinal bulb. In the adult, zfGLUT2 expression was highest in testis, brain, skin, kidney, and intestine, followed by liver and muscle. In the intestine, zfGLUT2 transcripts were detected in absorptive enterocytes, and its mRNA levels were altered by fasting and refeeding, suggesting that its expression in the intestine may be regulated by the nutritional status. These results indicate that the structure and function of GLUT2 has been remarkably well conserved during vertebrate evolution and open the way for the use of zebrafish as a model species in which to study the biology and pathophysiology of GLUT2.en_US
dc.description.sponsorshipThis study was supported by grants from the Ministerio de Educacio´n y Ciencia, Spain (CSD2007-0002) and the Xarxa de Refere`ncia de Recerca i Desenvolupament en Aqu¨icultura, Generalitat de Catalunya, Spain to J. V. Planas M. Diaz was supported by a fellowship from the Departament d’Universitats, Recerca i Societat de la Informacio´ (Generalitat de Catalunya).en_US
dc.format.extent22195 bytes-
dc.publisherAmerican Physiological Societyen_US
dc.subjectGene expressionen_US
dc.subjectGlucose transporten_US
dc.titleEvolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafishen_US
dc.description.peerreviewedPeer revieweden_US
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