English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/21345
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 0 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Evolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafish

AutorCastillo, Juan; Crespo, Diego; Capilla, Encarnación; Díaz, Mònica; Chauvigné, François ; Cerdà, Joan ; Planas, Josep V.
Palabras claveGene expression
Glucose transport
Affinity
Intestine
Fecha de publicación23-sep-2009
EditorAmerican Physiological Society
CitaciónThe American Journal of Physiology- Regulatory Integrative Comparative Physiology 297(5):1570-1581 (2009)
ResumenEvolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafish. Am J Physiol Regul Integr Comp Physiol 297: R1570–R1581, 2009. First published September 23, 2009; doi:10.1152/ajpregu.00430.2009.—In mammals, GLUT2 plays an essential role in glucose homeostasis. From an evolutionary perspective, relatively little is known about the biology of GLUT2, or other GLUTs, in nonmammalian vertebrates. Here, we have conducted studies to functionally characterize GLUT2 in zebrafish. First, we cloned the zebrafish ortholog of GLUT2 (zfGLUT2) encoding a protein of 504 amino acids with high-sequence identity to other known vertebrate GLUT2 proteins. The zfGLUT2 gene consists of 11 exons and 10 introns, spanning 20 kb and mapping to a region of chromosome 2 that exhibits conserved synteny with human chromosome 3. When expressed in Xenopus oocytes, zfGLUT2 transported 2-deoxyglucose (2-DG) with similar affinity than mammalian GLUT2 (Km of 11 mM). Transport of 2-DG was competed mostly by D-fructose and D-mannose and was inhibited by cytochalasin B. During early development, zfGLUT2 expression was detected already at 10 h postfertilization and remained elevated in 5-day larvae, when it was clearly localized to the liver and intestinal bulb. In the adult, zfGLUT2 expression was highest in testis, brain, skin, kidney, and intestine, followed by liver and muscle. In the intestine, zfGLUT2 transcripts were detected in absorptive enterocytes, and its mRNA levels were altered by fasting and refeeding, suggesting that its expression in the intestine may be regulated by the nutritional status. These results indicate that the structure and function of GLUT2 has been remarkably well conserved during vertebrate evolution and open the way for the use of zebrafish as a model species in which to study the biology and pathophysiology of GLUT2.
Descripción12 pages, 9 figures, 2 tables
Versión del editorhttp://dx.doi.org/10.1152/ajpregu.00430.2009
URIhttp://hdl.handle.net/10261/21345
DOI10.1152/ajpregu.00430.2009
ISSN1522-1490
Aparece en las colecciones: (ICM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.