Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/213387
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Arias, Armando | - |
dc.contributor.author | Perales, Celia | - |
dc.contributor.author | Escarmís, Cristina | - |
dc.contributor.author | Domingo, Esteban | - |
dc.date.accessioned | 2020-06-04T11:40:56Z | - |
dc.date.available | 2020-06-04T11:40:56Z | - |
dc.date.issued | 2010-05-20 | - |
dc.identifier | doi: 10.1371/journal.pone.0010735 | - |
dc.identifier | issn: 1932-6203 | - |
dc.identifier.citation | PLoS ONE 5 (2010) | - |
dc.identifier.uri | http://hdl.handle.net/10261/213387 | - |
dc.description.abstract | Background: Success of a viral infection requires that each infected cell delivers a sufficient number of infectious particles to allow new rounds of infection. In picornaviruses, viral replication is initiated by the viral polymerase and a viral-coded protein, termed VPg, that primes RNA synthesis. Foot-and-mouth disease virus (FMDV) is exceptional among picornaviruses in that its genome encodes 3 copies of VPg. Why FMDV encodes three VPgs is unknown. Methodology and Principal Findings: We have constructed four mutant FMDVs that encode only one VPg: either VPg1, VPg3, or two chimeric versions containing part of VPg1 and VPg3. All mutants, except that encoding only VPg1, were replication-competent. Unexpectedly, despite being replication-competent, the mutants did not form plaques on BHK-21 cell monolayers. The one-VPg mutant FMDVs released lower amounts of encapsidated viral RNA to the extracellular environment than wild type FMDV, suggesting that deficient plaque formation was associated with insufficient release of infectious progeny. Mutant FMDVs subjected to serial passages in BHK-21 cells regained plaque-forming capacity without modification of the number of copies of VPg. Substitutions in non-structural proteins 2C, 3A and VPg were associated with restoration of plaque formation. Specifically, replacement R55W in 2C was repeatedly found in several mutant viruses that had regained competence in plaque development. The effect of R55W in 2C was to mediate an increase in the extracellular viral RNA release without a detectable increase of total viral RNA that correlated with an enhanced capacity to alter and detach BHK-21 cells from the monolayer, the first stage of cell killing. Conclusions: The results link the VPg copies in the FMDV genome with the cytopathology capacity of the virus, and have unveiled yet another function of 2C: modulation of picornavirus cell-to-cell transmission. Implications for picornaviruses pathogenesis are discussed. © 2010 Arias et al. | - |
dc.description.sponsorship | BFU2008-02816/BMC from Ministerio de Ciencia e Innovacion (MICINN), and Fundacion R. Areces. CIBERehd (Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas) is funded by Instituto de Salud Carlos III | - |
dc.language | eng | - |
dc.publisher | Public Library of Science | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Deletion mutants of VPg reveal new cytopathology determinants in a picornavirus | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1371/journal.pone.0010735 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0010735 | - |
dc.date.updated | 2020-06-04T11:40:56Z | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.contributor.funder | Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.pmid | 20505767 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
DeletionMutants.pdf | 1,09 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
12
checked on 05-abr-2024
SCOPUSTM
Citations
20
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
17
checked on 26-feb-2024
Page view(s)
138
checked on 19-abr-2024
Download(s)
77
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.