Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/213213
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

A specific isoform of Pyd/ZO-1 mediates junctional remodeling and formation of slit diaphragms

AuthorsCarrasco-Rando, Marta CSIC; Prieto-Sánchez, Silvia; Culi, Joaquim CSIC ORCID; Tutor, Antonio S.; Ruiz-Gómez, Mar CSIC ORCID
Issue Date6-Jun-2019
CitationJournal of Cell Biology 218: 2294- 2308 (2019)
AbstractThe podocyte slit diaphragm (SD), responsible for blood filtration in vertebrates, is a major target of injury in chronic kidney disease. The damage includes severe morphological changes with destabilization of SDs and their replacement by junctional complexes between abnormally broadened foot processes. In Drosophila melanogaster, SDs are present in nephrocytes, which filter the fly's hemolymph. Here, we show that a specific isoform of Polychaetoid/ZO-1, Pyd-P, is essential for Drosophila SDs, since, in pyd mutants devoid of Pyd-P, SDs do not form and the SD component Dumbfounded accumulates at ectopic septate-like junctions between abnormally aggregated nephrocytes. Reintroduction of Pyd-P leads to junctional remodeling and their progressive normalization toward SDs. This transition requires the coiled-coil domain of Pyd-P and implies formation of nonclathrin vesicles containing SD components and their trafficking to the nephrocyte external membrane, where SDs assemble. Analyses in zebrafish suggest a conserved role for Tjp1a/ZO-1 in promoting junctional remodeling in podocytes.
Publisher version (URL)http://dx.doi.org/10.1083/jcb.201810171
URIhttp://hdl.handle.net/10261/213213
DOI10.1083/jcb.201810171
Identifiersdoi: 10.1083/jcb.201810171
issn: 1540-8140
Appears in Collections:(CBM) Artículos

Files in This Item:
File Description SizeFormat
Ruíz-GómezM_ASpecificIsoformOfPyd.pdf4,94 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.