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A Modular Vector Toolkit with a Tailored Set of Thermosensors to Regulate Gene Expression in Thermus thermophilus

AuthorsVerdú, Carlos; Sánchez Esther.; Ortega, Carmen; Hidalgo, Aurelio ; Berenguer, José ; Mencía, J. S.
Issue Date2019
PublisherACS Publications
CitationACS Omega (2019)
AbstractModular plasmid architectures have shown to be a very useful resource to standardize, build, share, and compare biological parts and functional vectors, and are being applied in an increasing number of microorganisms. Here, we present a modular plasmid toolkit for Thermus thermophilus, a species considered as a workhorse for biotechnology and a model for high-temperature biology. Apart from integrating improved versions of already existing parts, we have characterized specific promoters and developed a thermosensor-based palette that restricts the expression to Thermus and, at the same time, controls protein expression in this organism in a temperature-dependent manner. ©
Publisher version (URL)http://dx.doi.org/10.1021/acsomega.9b02107
Identifiersdoi: 10.1021/acsomega.9b02107
issn: 2470-1343
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