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Title

A selective culture medium for screening linezolid-resistant gram-positive bacteria

AuthorsNordmann, Patrice; Rodríguez-Villodres, Ángel; Poirel, Laurent
KeywordsLinezolid
Antibiotic
Screening
Enterococci
Staphylococci
Issue DateSep-2019
PublisherElsevier
CitationDiagnostic Microbiology and Infectious Disease 95(1): 1-4 (2019)
AbstractThe SuperLinezolid medium was developed for screening resistance to linezolid (LZD) in Gram-positive bacteria (Staphylococcus spp., Enterococcus spp.). It was evaluated using LZD-susceptible (n = 20) and LZD-resistant (n = 17) Gram-positive isolates. The sensitivity was found to be 82% at 24 h (3 out of 17 isolates being missed), and reached 100% at 48 h. At 48 h, a single LZD-susceptible isolate grew (specificity 95%). By testing stools spiked with LZD-resistant Gram-positive strains, an excellent performance of the medium was observed, with a lowest detection limit ranging from 101 to 102 CFU/ml. Overall, this medium is accurate for detection of LZD-resistant Gram-positive isolates after 24 h of culture.
Publisher version (URL)http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.006
URIhttp://hdl.handle.net/10261/213136
DOI10.1016/j.diagmicrobio.2019.03.006
ISSN0732-8893
E-ISSN1879-0070
Appears in Collections:(IBIS) Artículos
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