English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/21301
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 0 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL

Analysis of Vitelline Envelope Synthesis and Composition During Early Oocyte Development in Gilthead Seabream (Sparus aurata)

AutorModig, Carina; Raldúa, Demetrio; Cerdà, Joan ; Olsson, Per-Erik
Palabras claveEggshell
ZP ultrastructure
Fecha de publicaciónago-2008
EditorJohn Wiley & Sons
CitaciónMolecular Reproduction and Development 75(8): 1351-1360 (2008)
ResumenThe oocyte vitelline envelope (VE) of gilthead seabream is composed of four known zona pellucida (ZP) proteins, ZPBa, ZPBb, ZPC, and ZPX. We have previously shown that the gilthead seabream ZP proteins are differentially transcribed in liver and ovary, with the expression in liver being under estrogenic control. However, although mRNA was found in both liver and ovary, only low ZPBa protein levels were detected in liver and plasma. Using isoform-specific ZP antibodies we show that ZPBa and ZPX translation products are present in the cytosol of stage I and II oocytes. In addition, the zpBa and zpX mRNAs were detected in early developing oocytes. During oocyte growth (vitellogenesis), the VE increased in thickness (>10 mm), and we show that the four ZP isoforms are present in different regions of the VE. ZPX was detected closest to the oocyte plasma membrane while the intermediate region was composed of ZPBa, ZPBb, and ZPC. At the outer layer, only ZPC was detected. When oocytes reach the fully grown stage they resume meiosis and hydration. As the oocyte expands, thinning to 4 mm, the VE acquire a striped and compact appearance at the electron microscopy level. This study provides further evidence for the oocyte origin of some ZP proteins in the gilthead seabream and suggests that the ZP proteins are differentially distributed within the VE.
Descripción10 pages,7 figures,1 table
Versión del editorhttp://dx.doi.org/10.1002/mrd.20876
Aparece en las colecciones: (ICM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.