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Title

Use of liquid medium and biofortificants for improving micropropagation and acclimation of Musa AAA cv. Williams

AuthorsMoreno A; Bernal, A; Ugarte F; Lima K; Coig M; Sánchez C ; Aldrey A; Vidal N
Issue Date10-Sep-2018
AbstractThe aim of this study was to develop alternative methods both for the micropropation of banana cv. Williams and for the acclimation of the obtained plantlets. In Ecuador, banana production is being threatened by the age of plantations and diseases like Mycosphaerella fijiensis Morelet. Although bananas are traditionally propagated by suckers, by using tissue-cultured plants it is possible to produce larger quantities of healthy plantlets for both small- and large-scale farmers. In this study we present three sets of results i) in vitro establishment of genotypes of banana growing at ¿El Oro¿ (Ecuador), ii) micropropagation of banana in liquid medium by temporary immersion, and iii) use of extracts of Moringa oleifera as a biofortificant during the acclimation of banana vitroplants. For in vitro establishment, we used lateral shoots induced in mother plants grown in a greenhouse and previously disinfected with fungicides and insecticides. The shoots were treated with detergents, peeled, treated with sodium hipoclorite, peeled, treated with cystein for elimination of phenolics and peeled again to a size of 8 mm diameter and 10 mm height. These explants were inoculated on paper brigdes over liquid medium Murashige and Skoog with 2 mg L-1 N6-benziladenine and 0.023 mg L-1 indole-3-acetic-acid. For the micropropagation of banana in liquid medium we used plant material previously established in vitro by CULTESA in Spain and genotypes established in vitro in Ecuador. Explants were propagated in commercial bioreactors RITA® and plantform¿, as well as in bioreactors of the two-flask system adapted from glass bottles. For acclimation experiments, we used banana vitroplants produced by Orangelab (Quito, Ecuador), and acclimated for 3 weeks in a greenhouse. These plantlets were sprayed for other 3 weeks with extracts of different concentrations obtained from juvenile and mature leaves of M. oleifera, a tree with high biotechnology potential as growth enhancer and plant defense promoter. The obtained results indicate: i) the need to follow extremely careful sterilization protocols for establishing banana cultures, in order to counteract the presence of fungal and bacterial contaminants due to the climate conditions of ¿El Oro¿ (Ecuador), ii) the potential of temporary immersion systems for propagation of banana, and iii) the beneficial effects of extracts of moringa leaves on vitroplants of banana during the acclimation step.
URIhttp://hdl.handle.net/10261/212729
Appears in Collections:(IIAG) Comunicaciones congresos
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