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A general HPTLC detection of analytes, including non-fluorescent ones, by changes in fluorescent emission of coralyne cation

AuthorsMateos Serrano, Elena; Cebolla, Vicente L. ; Gálvez Buerba, Eva Mª ; Membrado, Luis; Cossío, Fernando P.; Matt, Muriel
Fluorescence scanning densitometry
Planar chromatography
Issue Date11-Oct-2006
CitationHPTLC 2006
AbstractMany chemical compounds, including non-fluorescent ones, induce changes (either increase in emission or quenching) in the fluorescence spectra of coralyne cation, in absence and presence of solvent. We have applied this phenomenon to HPTLC with fluorescence scanning densitometry, using coralyne as a revealing agent, as a general detection system for analytes. It does not involve covalent interactions. Alkanes in presence of coralyne provide an enhancement of the fluorescent signal, which depends on alkane concentration and structure, when the system is irradiated with UV light. Responses of compounds other than alkanes are structure-dependent.
DescriptionWork presented at the International Symposium for TLC, HPTLC 2006, 9th-11th October, Berlin (Germany)
Publisher version (URL)https://www.hptlc.com/version-2016/pdf/2006pdf/abstract/A_general_HPTLC_detection_cebolla.pdf
Appears in Collections:(ICB) Comunicaciones congresos
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