English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/212527
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Encapsulation of the Antistaphylococcal Endolysin LysRODI in pH-Sensitive Liposomes

AuthorsPortilla Vázquez, Silvia; Fernández, Lucía; Gutiérrez, Diana ; Rodríguez, Ana ; García, Pilar
KeywordsEndolysins
Staphylococcus aureus
Liposomes
Issue Date9-May-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationAntibiotics 9(5): 242 (2020)
AbstractPhage lysins are promising new therapeutics against multidrug-resistant bacteria. These so-called enzybiotics offer, amongst their most notable advantages, high target specificity and low resistance development. Moreover, there are numerous recent and ongoing studies aimed at demonstrating the efficacy and safety of endolysins in animal models or even in clinical trials. Nonetheless, as is the case for other antimicrobials, it is important to assess potential strategies that may broaden their potential applications or improve their stability. Encapsulation, for instance, has given very good results for some antibiotics. This study sought to evaluate the feasibility of encapsulating an endolysin against the opportunistic human pathogen Staphylococcus aureus, one of the most problematic bacteria in the context of the current antibiotic resistance crisis. Endolysin LysRODI has antimicrobial activity against many S. aureus strains from different sources, including methicillin-resistant S. aureus (MRSA) isolates. Here, this protein was encapsulated in pH-sensitive liposomes with an efficacy of approximately 47%, retaining its activity after being released from the nanocapsules. Additionally, the encapsulated endolysin effectively reduced S. aureus cell counts by > 2log units in both planktonic cultures and biofilms upon incubation at pH 5. These results demonstrate the viability of LysRODI encapsulation in liposomes for its targeted delivery under mild acidic conditions
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/antibiotics9050242
URIhttp://hdl.handle.net/10261/212527
DOI10.3390/antibiotics9050242
E-ISSN2079-6382
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
Encapsulation_Portilla_Art2020.pdf1,12 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.