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Analyzing RNA polymerase III by electron cryomicroscopy

AuthorsFernández-Tornero, Carlos ; Böttcher, Bettina; Rashid, Umar J.; Müller, Christoph W.
KeywordsTranscriptional regulation
RNA polymerase III
Electron cryomicroscopy
Transcription elongation
Transcription initiation
Issue Date1-Sep-2011
PublisherTaylor & Francis
CitationRNA Biol 8 (5) 760–765 (2011)
AbstractRecent electron cryomicroscopy reconstructions have provided new insights into the overall organization of yeast RNA polymerase (Pol) III, responsible for the synthesis of small, non-translated RNAs. The structure of the free Pol III enzyme at 10 Å resolution provides an accurate framework to better understand its overall architecture and the structural organization and functional role of two Pol III-specific subcomplexes. Cryo-EM structures of elongating Pol III bound to DNA/RNA scaffolds show the rearrangement of the Pol III-specific subcomplexes that enclose incoming DNA. In one reconstruction downstream DNA and newly transcribed RNA can be followed over considerably longer distances as in the crystal structure of elongating Pol II. The Pol III transcription machinery is increasingly recognized as a possible target for cancer therapy. The recent cryo-EM reconstructions contribute to the molecular understanding of Pol III transcription as a prerequisite for targeting its components.
Description6 p.-2 fig.
Publisher version (URL)https://doi.org/10.4161/rna.8.5.16021
Appears in Collections:(CIB) Artículos
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