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dc.contributor.authorKinge, T. R.es_ES
dc.contributor.authorCason, Errol D.es_ES
dc.contributor.authorValverde Portal, Ángeles_ES
dc.contributor.authorNyaga, M.es_ES
dc.contributor.authorGryzenhout, M.es_ES
dc.identifier.citationMycosphere 10(1): 739–756 (2019)es_ES
dc.description18 páginas, 6 figuras, 2 tablases_ES
dc.description.abstractPathogen-free seeds are important for the establishment of young seedlings, prevention of health problems upon consumption by animals and livestock, and inadvertent movement of pathogens into and out of countries. However, testing for the presence of fungi, including pathogens from seeds is a time consuming and difficult process. In this study, we characterized the seed fungal microbiome (mycobiome) of six commercial sorghum cultivars from South Africa using a deep amplicon next generation sequencing approach based on the Internal Transcribed Spacer (ITS) region of the ribosomal operon. Sorghum is the fifth most important crop in the world, and widely used by African farmers. We found that the fungi present in each of the seedlots were similar to those reported by cultural studies. By comparing phylotypes of certain key families and genera to phylotypes used in established phylogenies and reputed sequences from public databases, the diagnostic value of the NGS method was also investigated. We showed that a number of molecular operational taxonomic units (MOTUs) could be identified at the species level and established that certain known pathogens are not present in the tested seeds, for instance in the Aspergillus group. Other groups could not be identified, not even to genus level. While acknowledging the shortcomings of using partial ITS data, we demonstrated that deep amplicon sequencing is a valuable diagnostic tool for seed disease control and prevention in some cases.es_ES
dc.description.sponsorshipResearch funding was provided by the Agricultural Research Council Collaborative Consortium to Broaden the Food Base.es_ES
dc.publisherGuizhou Academy of Agricultural Scienceses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectDeep amplicon sequencinges_ES
dc.subjectEndophytic Fungies_ES
dc.subjectSeedborne fungies_ES
dc.titleEndophytic seed mycobiome of six sorghum (Sorghum bicolor) cultivars from commercial seedlots using an Illumina sequencing approaches_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderAgricultural Research Council (South Africa)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidValverde Portal, Ángel [0000-0003-0439-9605]es_ES
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