English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/212340
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Immunological homeostasis at the ovine placenta may reflect the degree of maternal fetal interaction

AuthorsWattegedera, S.; Doull, L. E.; Goncheva, M. I.; Wheelhouse, N. M.; Watson, D. M.; Pearce, J.; Benavides, Julio ; Palarea-Albaladejo, Javier; McInnes, C. J.; Ballingall, K.; Entrican, G.
KeywordsOvine
Major histocompatibility complex
Indoleamine 2,3 dioxygenase-1
Synepitheliochorial placentation
Trophoblast
Issue Date2019
PublisherFrontiers Media
CitationFrontiers in Immunology 9: 3025 (2019)
AbstractSuccessful mammalian pregnancies are a result of complex physiological, endocrinological, and immunological processes that combine to create an environment where the mother is tolerant to the semi-allogeneic fetus. Our knowledge of the mechanisms that contribute to maternal tolerance is derived mainly from human and murine studies of haemochorial placentation. However, as this is the most invasive type of placentation it cannot be assumed that identical mechanisms apply to the less invasive epitheliochorial placentation found in other species such as ruminants. Here, we examine three features associated with reproductive immune regulation in a transformed ovine trophoblast cell line and ex-vivo ovine reproductive tissues collected at term, namely: major histocompatibility complex (MHC) expression, Indoleamine 2,3 dioxygenase-1 (IDO-1) expression, and Natural Killer (NK) cell infiltration. High levels of MHC class I protein expression were detected at the surface of the trophoblast cell line using a pan-MHC class I specific monoclonal antibody. The majority of MHC class I transcripts isolated from the cell line clustered with classical MHC alleles. Transcriptional analysis of placental tissues identified only classical MHC class I transcripts. We found no evidence of constitutive transcription of IDO-1 in either the trophoblast cell line or placental tissues. Ex-vivo tissues collected from the materno-fetal interface were negative for cells expressing NKp46/NCR1. Collectively, these observations suggest that the relatively non-invasive synepitheliochorial placentation found in sheep has a more limited requirement for local immunoregulation compared to the more invasive haemochorial placentation of primates and rodents.
Description10 páginas, 4 figuras.
Publisher version (URL)http://dx.doi.org/10.3389/fimmu.2018.03025
URIhttp://hdl.handle.net/10261/212340
DOIhttp://dx.doi.org/10.3389/fimmu.2018.03025
Identifiersdoi: 10.3389/fimmu.2018.03025
issn: 1664-3224
Appears in Collections:(IGM) Artículos
Files in This Item:
File Description SizeFormat 
fimmu-09-03025.pdf1,48 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.