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Title

Transcriptome Analysis of the European Sea Bass (Dicentrarchus labrax) using a Custom-made Microarray

AuthorsSánchez Baizán, Núria
AdvisorPiferrer, Francesc ; Ribas, Laia
KeywordsMicroarray
Dicentrarchus labrax
Transcriptome
Gonadal development
Ovarian steroidogenesis
Fatty acids
Issue DateApr-2015
AbstractThe European sea bass (Dicentrarchus labrax) is an economically important farmed species in European countries of which demand is rapidly increasing throughout the planet. Nevertheless, male-biased populations are still a problem for production under captivity because males grow about 35% smallert han females. In order to determine differential gene expression (DGE) profiles between ovaries and testis, a custom-made microarray was designed to analyze the gonadal transcriptome of the European sea bassat 350 days post fertilization (dpf1). After experiment validation, a total of 43,803 readings were obtained. The statistical analysis of the data set indicated 844 genes to be differentially expressed, of which 719 were down-regulated and 125 genes were up regulated in ovaries. The expression profile of significantly differentially expressed genes involved in ovarian steroidogenesis and steroid hormone biosynthesis were mapped to biochemical pathways. The sexual dimorphic expression of sex-related genes provides novel information to the understanding of the last stages of gonadal differentiation and adult gonadal development of the sea bass. The present analysis also generated a list of gene candidates for further investigation to elucidate molecular mechanisms of reproduction; such as the proposed set of genes with potential correlation tosteroidogenic genes which could regulate an increasedfatty acid synthesis in the gonads
DescriptionTrabajo final presentado por Núria Sánchez Baizán para obtener el Marine Science Degree, realizado bajo la dirección del Dr. Francesc Piferrer y de la Dra. Laia Ribas del Institut de Ciències del Mar (ICM-CSIC).-- 29 pages, 5 figures, 3 tables
URIhttp://hdl.handle.net/10261/211935
Appears in Collections:(ICM) Tesis
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