English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/211745
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Structures of the Leishmania infantum polymerase beta

AuthorsMejía Edison; Burak, Matthew; Alonso, Ana ; Larraga, Vicente ; Kunkel, Thomas A.; Bebenek, Katarzyna; García-Díaz, Miguel
KeywordsDNA repair
Leishmaniasis
DNA polymerase
Family X
Issue DateJun-2014
PublisherElsevier
CitationDNA Repair (Amst) 18:1-9 (2014)
AbstractProtozoans of the genus Leishmania, the pathogenic agent causing leishmaniasis, encode the family X DNA polymerase Li Pol β. Here, we report the first crystal structures of Li Pol β. Our pre- and post-catalytic structures show that the polymerase adopts the common family X DNA polymerase fold. However, in contrast to other family X DNA polymerases, the dNTP-induced conformational changes in Li Pol β are much more subtle. Moreover, pre- and post-catalytic structures reveal that Li Pol β interacts with the template strand through a nonconserved, variable region known as loop3. Li Pol β Δloop3 mutants display a higher catalytic rate, catalytic efficiency and overall error rates with respect to WT Li Pol β. These results further demonstrate the subtle structural variability that exists within this family of enzymes and provides insight into how this variability underlies the substantial functional differences among their members.
Description9 p.-4 fig.-4 tab.
Publisher version (URL)https://doi.org/10.1016/j.dnarep.2014.03.001
URIhttp://hdl.handle.net/10261/211745
DOI10.1016/j.dnarep.2014.03.001
ISSN1568-7864
E-ISSN1568-7856
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdfRestringido15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.