English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/211743
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Proteome profiling of the growth phases of Leishmania pifanoi promastigotes in axenic culture reveals differential abundance of immunostimulatory proteins

AuthorsAlcolea, Pedro J. ; Alonso, Ana ; García-Tabares, Francisco ; Mena, M. Carmen; Ciordia, Sergio; Larraga, Vicente
KeywordsLeishmania pifanoi
Differential gene expression
2DE
MALDI-TOF/TOF
Elongation factor 1β
Tryparedoxin peroxidase
hsp70
Enolase
Aldehyde dehydrogenase
Issue DateJun-2016
PublisherElsevier
CitationActa Tropica 158: 240- 247 (2016)
AbstractLeishmaniasis is a term that encompasses a compendium of neglected tropical diseases caused by dimorphic and digenetic protozoan parasites from the genus Leishmania (Kinetoplastida: Trypanosomatidae). The clinical manifestations of neotropical cutaneous leishmaniasis (NCL) caused by Leishmania pifanoi and other species of the “Leishmania mexicana complex” mainly correspond to anergic diffuse cutaneous leishmaniasis (ADCL), which is the origin of considerable morbidity. Despite the outstanding advances in the characterization of the trypanosomatid genomes and proteomes, the biology of this species has been scarcely explored. However, the close relation of L. pifanoi to the sequenced species L. mexicana and others included in the “L. mexicana complex” allowed us to perform a two-dimension electrophoresis (2DE) approach to the promastigote proteome at the differential expression level. Protein identifications were performed by matrix-assisted laser desorption-ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF). This insight has revealed similarities and differences between L. pifanoi and other species responsible for cutaneous and visceral leishmaniasis. Interestingly, certain proteins that were previously described as immunostimulatory (elongation factor 1β, trypanothione peroxidase, heat shock protein 70, enolase, GDP-forming succinyl-CoA and aldehyde dehydrogenase) are more abundant in the final growth stages of promastigotes (late-logarithmic and/or stationary phase) in the case of L. pifanoi.
Description31 p.-4 fig.-2 tab.-1 graph. abst.
Publisher version (URL)https://doi.org/10.1016/j.actatropica.2016.03.015
URIhttp://hdl.handle.net/10261/211743
DOI10.1016/j.actatropica.2016.03.015
ISSN0001-706X
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
acta tropica_alcolea_2016.pdfPostprint876,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.