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Title: | SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
Authors: | Randazzo, Walter; Truchado, Pilar ![]() ![]() ![]() |
Keywords: | Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus |
Issue Date: | 16-May-2020 |
Publisher: | Elsevier |
Citation: | Water Research 181: 115942 (2020) |
Abstract: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 ± 3.5% and 10 ± 2.1% in influent water (n = 2) and 3.3 ± 1.6% and 6.2 ± 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater waters of 5.4 ± 0.2 log10 genomic copies/L on average. Two secondary water samples resulted positive (2 out of 18) and all tertiary water samples tested as negative (0 out 12). This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that members of the community were shedding SARS-CoV-2 RNA in their stool even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. |
Description: | Preprint disponible en http://hdl.handle.net/10261/209125 |
Publisher version (URL): | https://doi.org/10.1016/j.watres.2020.115942 |
URI: | http://hdl.handle.net/10261/211707 |
DOI: | 10.1016/j.watres.2020.115942 |
ISSN: | 0043-1354 |
E-ISSN: | 1879-2448 |
Appears in Collections: | (CEBAS) Artículos (VICYT) Colección Especial COVID-19 (IATA) Artículos |
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File | Description | Size | Format | |
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Randazzo_Manuscript.pdf Embargoed until May 16, 2022 | Postprint | 285,42 kB | Adobe PDF | ![]() View/Open Request a copy |
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