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The Piwi pathway in Blattella germanica ovaries

AuthorsPaniello O.; Montañés, J. C.; Pagone, Viviana ; Piulachs, Maria-Dolors
Issue Date2019
CitationEight International Symposium on Molecular Insect Science (2019)
AbstractOogenesis, the formation and maturation of an oocyte, is a crucial process in the female reproductive physiology in animals. It encompasses a series of events that must occur in the correct order and at the right time. Hormonal signals are fundamental in the oogenesis processes, for oocyte differentiation and growth, to determine changes in cell program or for chorion synthesis. However, the maintenance of genome stability in insect germ cells during the proliferation and differentiation is of paramount importance, and it is the Piwi pathway which plays this role. Using Blattella germanica as a model, a species representative of panoistic ovaries, we studied three of the core proteins of the Piwi pathway in the ovaries, in order to illuminate their function in the maintenance of germ cells stability. B. germanica transcriptomes and bioinformatic analyses were used to identify the main proteins involved in the biogenesis of piRNAs and its regulation. By quantitative real-time PCR, the expression of Piwi, Argonaute 3 (Ago3) and Yb (fs(1)Yb or Tudor 12) was analyzed in B. germanica ovaries, and their functions were studied by RNAi. Piwi, Ago3 and Yb, are mainly expressed in ovaries of last nymphal instar when the differentiation of germinal cells is usually arrested. The expression of these genes is maintained during the first gonadotrophic cycle. Depletion of Yb and Ago3 allowed us to demonstrate their involvement in the primary and secondary piRNAs pathway, respectively.
DescriptionPoster presented at the Eight International Symposium on Molecular Insect Science, held 7-10th July 2019 in Barcelona (Spain).
Appears in Collections:(IBE) Comunicaciones congresos
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