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Impact of optical tissue clearing on the brillouin signal from biological tissue samples

AuthorsJiménez Riobóo, R. J. ; Desco, Manuel; Gómez-Gaviro, M.Victoria
KeywordsBiological tissues
Mechanical properties
Brillouin spectroscopy
Issue Date2019
PublisherOptical Society of America
CitationBiomedical Optics Express 10: 2674- 2683 (2019)
Abstract[EN] Brillouin spectroscopy is a well-established technology in condensed matter physics to characterize the mechanical properties of inert materials, and it has been extended very recently to the study of biological samples. Transparency is beneficial for samples to be properly analyzed by Brillouin spectroscopy. Here, we explored the efficacy of optical tissue clearing techniques to improve the acquisition of Brillouin spectra from biological tissues in order to analyze their biomechanical properties. We describe the first application of Brillouin scattering to optically cleared biological tissues with CUBIC protocol. We conclude that, within the range of error, tissue clearing does not modify the mechanical properties of the studied biological tissues.
Publisher version (URL)http://dx.doi.org/10.1364/BOE.10.002674
Identifiersdoi: 10.1364/BOE.10.002674
e-issn: 2156-7085
Appears in Collections:(ICMM) Artículos
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